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Leica
AM 6000
Advanced
Micromanipulation
Transgenetic Platform
顯微基因轉殖操作與注射系統
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Leica
AM 6000 一般應用範疇
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| Transgenic |
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PGD
( Preimplantation genetic Diagnosis ) |
| Pronuclear
Microinjection |
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IVF
( In-Vitro Fertilization ) |
| Embryonic
Stem Cell transfer |
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ICSI
( Intracytoplasmic sperm injection ) |
| Tissue
engineering and somatic gene therapy |
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IMSI
( Intracytoplasmic morphological selected
sperm injetion ) |
| Microinjection
and micromanipulation |
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Assisted
hatching |
| Polarbody
biopsy |
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Blastomere biopsy |
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| 注射系統
Microinjection
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| CellTram |
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QuickValve
system
Filling CellTram
vario and CellTram Oil is quick and
easy with the new QuickValve system.
A
special valve screw allows the oil to
fill directly into the piston chamber.
The optimized piston/cylinder system
minimizes friction in the piston
chamber, thereby ensuring the utmost
reliability and ultra-fine
sensitivity.
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| 訂購參考資料 |
| 標準配備
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CellTram |
| CellTram™
Air, pneumatic manual microinjector,
complete with pressure tube and
universal capillary holder with grip
head for capillaries with an outer
diameter of 1 mm |
| CellTram™
Oil, hydraulic manual microinjector,
complete with pressure tube, filling
tube, oil and universal capillary
holder with grip head for capillaries
with an outer diameter of 1 mm |
| CellTram™
vario, hydraulic manual microinjector,
with gears, complete with pressure
tube, filling tube, syringe, oil, and
universal capillary holder with grip
head for capillaries with an outer
diameter of 1 mm |
| 選配配件 |
| 2 x
Grip Head 0 for microcapillaries;
outer diameter 1.0 mm-1.1 mm |
| 2 x
Grip Head 2 for microcapillaries;
outer diameter 1.4 mm-1.5 mm |
| 2 x
Grip Head 3 for microcapillaries;
outer diameter 0.7 mm-0.9 mm |
| Pressure
Tube, for CellTram™, 1 m |
| O-rings,
for microinjectors, pkg. of 10 |
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| 注射系統
Microinjection
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| FemtoJet |
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FemtoJet |
FemtoJet
express |
| 圖示 |
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| 應用
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Semiautomatic
injection into adherent cells with
InjectMan NI 2 |
Microinjection
into small organisms or embryos at
early developmental stages: e.g.,
drosophila melanogaster, xenopus
laevis, Caenorhabditis elegans (C.
elegans), zebrafish, medaka, etc. |
| Suspension
cell injection with TransferMan NK 2 |
Pronuclear
injections into mouse embryos |
| Pronuclear
injections into mouse embryos |
Serial
injection volumes from 100 picoliters
up to 1 microliter |
| Serial
injection volumes from femtoliter up
to 100 picoliters |
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| 主要設計 |
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Programmable
injection ensures reproducibility
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Injection volumes
range from femtoliters to
microliters
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User-friendly
interface with large LCD display
for easy operation
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Additional
menu-controlled functions (e.g.,
for bubble pressure test)
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Inject and Clean
functions triggered via control
button on instrument, supplied
mouse or optional foot control
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Electronic
linkage to InjectMan NI 2
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Easily combined
with all common micromanipulation
systems
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| Pressure
supply: |
integrated
compressor |
external
pressure source |
| Injection
Time (ti): |
0.0
to 99.9 sec; can be set in increments
of 0.1 sec |
| Injection
Pressure (pi): |
0;
15 hPa-max. 6,000 hPa (87 psi); can be
set in increments of 1 hPa |
| Compensation
Pressure (pc): |
0;
15 hPa-max. 6,000 hPa (87 psi); can be
set in increments of 1 hPa |
| Rinsing
Pressure : |
Maximum
7,000 hPa (102 psi) |
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| 訂購參考資料 |
| FemtoJet,
programmable microinjector with
integrated pressure supply, supplied
with grip head |
| FemtoJet
express, programmable microinjector
with external pressure supply |
| 選配配件 |
| Foot
Pedal, for FemtoJet®, used for
triggering Inject function |
| 2 x
Grip Head 0 for microcapillaries;
outer diameter 1.0 mm-1.1 mm |
| 2 x
Grip Head 2 for microcapillaries;
outer diameter 1.4 mm-1.5 mm |
| 2 x
Grip Head 3 for microcapillaries;
outer diameter 0.7 mm-0.9 mm |
| Injection
Tube, for FemtoJet®, 2 m |
| O-rings,
for microinjectors, pkg. of 10 |
| Pressure
tube for FemtoJet express, 2.5 m,
includes coupling G, 1/4 in |
注射量比較 |
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| 微調器
Micromanipulator
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| TransferMan
NK2 |
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| 主要設計 |
- Improved
proportional kinetics for
intuitive control of manipulation
- Menu-driven programming with
storage of user profiles
- Small steps for fine movement
- High maximum speed for rapid
work
- Storage facility for three
independent work positions
- Integrated coarse and fine
manipulator with a working range
of >20 mm per axis
- Programmable Z-axis limit for
defined injection level avoids
capillary breakage
- Automated Home function for
rapid capillary exchange
- Can be adapted to all common
microscopes
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應用 |
- Micromanipulation of suspension
cells
- ICSI
- PGD
- Pronuclear injection and ES
transfer
- Nuclear transfer
- Microdissection
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| Control: |
Via
central joystick; proportional
kinetics |
| Operating
range, X,Y,Z |
>20
mm each |
| Resolution,
X,Y,Z |
40
nm per |
| Stepper
motor:
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40
nm per microstep |
| Max.
speed of tool during injection: |
7.500
µm/sec. |
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| 訂購參考資料 |
| TransferMan® NK
2, micromanipulator |
| 選購配件
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| TwinTip-Holder, for
TransferMan® NK 2 micromanipulator |
| Microscope Adapter,
for Leica® DMIL/HC |
| Microscope Adapter,
for Leica® DMIRB/E/HC |
| Microscope Adapter,
for Leica® DMIRE2 |
| Universal Stand, for
all other microscopes |
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For special manipulation
techniques (e.g. biopsy) and for the
independent control of two capillaries on
one TransferMan NK2 |
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| Piezo
Drill |
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- The first efficient mouse intracytoplasmic
sperm injection (ICSI)
Kimura and Yanagimachi (1995). Biology of
Reproduction 52, 709-720.
- The first report of mouse cloning using
adult derived cells.
Wakayama et al. (1998). Nature 394,
369-374.
- The first mammalian transgenesis by
injecting an unfertilized oocyte (metaphase II
transgenesis)
Perry et al. (1999). Science 284,
1180-1183.
- The first report of pig cloning using adult
derived cells.
Onishi et al. (2000). Science 289,
1188-1190.
- The first comprehensive evaluation of
nuclear transfer ES cells - ntES cells.
Wakayama et al. (2001). Science 292,
740-743.
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- Micromanipulation of suspension cells
- ICSI
- Enucleation
- Nuclear transfer
- ES cell transfer
- Assisted hatching
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Components
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Functions
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Specifications
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Controller
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Output: 1
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240 (W) x 340 (D) x 140 (H) mm; weight
approx. 5.0kg
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Piezo Impact Drive Unit [MB-U]
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Moving range: approx. [plus or minus sign]
5mm
Traveling speed: 0.04mm/s max
Traveling resolution: approx. 0.1µm
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MB-U Impact Unit: 125g
(including wire)
MB-U Slide base unit: 75g (not including
mounting adapter)
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Operation box
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Two adjustment dials: 16 speed levels, 16
intensity levels
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100 (W) x 160 (D) x 60 (H) mm; weight
approx. 530g (including wire)
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Foot switch
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Arrow pedal to move left
Pedal selection indicator
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145 (W) x 140 (D) x 38 (H) mm; weight
approx. 820g (including wire)
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Manual injector [HDJ-M3]
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Syringe volume: 1.9ml
Screw sending pitch: 0.05mm
Screw sending stroke: 20mm
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70(W) x 182.5 (D) x 90 (H) mm; weight
approx. 0.88kg
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| 訂購參考資料 |
- PMM Controller (PMAS-CT150)
- Operation box (OP-15)
- Foot switch (OP-14)
- Piezo impact drive unit (MB-U)
- Manual injector (HDJ-M3)
- Mounting adapter
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| Nanoinjector
簡易微量注射裝置
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| Nanoject
II ( Auto Nano-liter Injector ) |
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- Automated Microprocessor
controlled Microinjection Pipet delivering
precise nanoliter volumes accurately
- Remote control and
nonrotating plunger eliminates vibration
allowing precise injection minimizing possible
cell rupture
- Selectable Injection Volumes:
from 2.3 nanoliters to 69.0 nanoliters in 16
increments
- Supplied with sufficient
N-51-A glass capillaries for preparing 400
injection tips, standard softening point 780°C
- Can be mounted on most
micromanipulators (Note: May require Universal
Adapter 3-000-024-A)
- Separate Foot Switch
available to allow hands-free operation
- Selectable Operating Speeds:
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Fast
speed |
Slow
speed |
| Fill Speed |
46 nL per second |
23 nL per second |
| Injection Speed |
46 nL per second |
23 nL per second |
| Empty Speed |
230 nL per second |
92 nL per second |
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