徠卡顯微鏡系統
雷射掃描共軛焦顯微鏡
半導體及工業用顯微鏡
顯微數位影像系統
細胞基因影像分析系統
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Leica EL 6000

光纖傳導 ( Gel Liquid Light Guide, 2 m Length, 3 mm Diameter ) - LEICA 光纖比一般光纖提供更高的光學穿透效率. ( 下圖之綠色曲線 )

   
燈管 HXP120W45C VIS Metal-Halogenide Lamp 燈源, 120 瓦功率 / 2000 小時
燈源平均使用壽命 可達 2000 小時, 最佳使用投資效益. ( 依測試, 當使用 3000 hours 後, 仍有 50% 的輸出效率 ). 平均一支燈管的使用壽命約等於 7 支 HBO 燈管. 最符合經濟效益.
無須調準燈管 更換燈泡, 無須做任何調整. 
衰減控制 5 段式衰減, 0%, 12.5%, 25%, 50%, 100%. ( 可配合 Leica IFW 及 FIM 控制 )
藍光與綠光激發光譜 EL 6000 在藍光與綠光激發光譜, 具有比 Xenon 或 HBO 更好的激發效率. 最適宜 FITC/GFP 及 Rhodamine 的應用.
光譜控制 可選配安裝螢光濾鏡轉盤 ( Filter wheel )
快門控制 內建快門控制, 切換速率高達 4 msec.
應用 多重光譜應用
可適用顯微鏡種類 可適用於 LEICA 所有光學顯微鏡級實體顯微鏡
可適用其他廠牌顯微鏡 ( 配合特定安裝固定座 )
基座特性 無熱, 安靜無噪音.
 
Leica EL 6000 內置  : IFW ( Integrated Fast Filterwheel )

高速螢光轉盤切換控制器 - 內建於顯微鏡螢光光路上, 提供高速的濾鏡切換速率, 遠低於 0.05 sec. 此外, 本公司也提供外接 Filterwheel ( Sutter Lambda  series, LUDL filterwheel, and Vincent UniBlitz shutter )

Fluorescence Intensity Manager ( FIM ) :    自動螢光照明強度管理系統,

  • 採用 5 段減光控制 ( 100%, 55%, 35%, 20%, 10% ), 避免螢光漂白. 可以保護細胞樣品,

  • 可以設定儲存, 使每一個螢光濾鏡, 每一個螢光染劑, 每一種樣品使用時, 皆能取得最佳激發光源的照明強度., 使螢光影像最佳化.

  • 依照所選用螢光濾鏡, 自動取得最佳激發光源的照明強度,  調整光源強度時, 不會影像對比效果.

  • 內建針孔 ( Pinhole ) 視野光圈.

  • 電控光路切換 ( 穿透光 與 螢光 )

Excitation Manager   多重螢光激發識別管理系統

  • 可調整不同螢光波長的螢光強度, 取得最佳平衡顯示效果.

  • 無螢光重疊現象 ( Cross-talk ), FITC / Texas Red. 

  • 汞燈 100W 的最佳光源管理 , 增加快門速率 0.1 sec, 增加濾鏡旋轉速率. 0.5 sec

 

 
 

The Lambda DG-4/DG-5 is a complete illumination system offering speed and versatility for experiments requiring rapid wavelength switching. The instrument retains all the advantages of interference filter based systems, yet eliminates the temporal constraints imposed by traditional filter changing devices like filter wheels. Switching between any two wavelengths is achieved in less than the 1.2msec vertical retrace period of a video signal, allowing the user to perform real-time video imaging. The high switching speed of the Lambda DG-4/DG-5 facilitates the ability to follow fast changes in ion concentrations in dual wavelength ratio imaging applications and to monitor other concomitant changes in the studied system at additional wavelengths.

超高速光波切換光源器 ( Dual Galvo driven wavelength switcher ), 光纖傳導 175W Xenon, 1.2 msec 的波長切換速率, 500 µs 的快門開關速率, 4 或 5 組內置選配螢光濾鏡, 選配減光濾鏡. 比單光儀的切換速率快 1000 倍以上, 最適宜做螢光離子流及高速動態的活細胞影像擷取與觀察.

Output Range
330nm to 700nm - Ozone free   /    300nm to 700nm - Full spectrum
Xenon Lamp Type
175 Watt, pre-aligned, ozone free   (175W full spectrum available upon request)
Lamp Expected Lifetime
1,000 hours    (All bulbs warranted for 500 hours. Longer life depends on your application.)
Power Consumption
350 Watts
Filter Diameter
DG-4: Four, 25mm (1inch) /   DG-5: Two, 25mm (1inch) and  Three 18mm

The newest member of our optical product family, the Lambda XL, is a broad spectrum, highly stable light source (±1% peak-to-peak fluctuations) with an average lamp lifetime of 10,000 hours.  The connection to the microscope is made through a liquid light guide, which assures output uniformity in the field of view. Two light guide options are available, one optimized for the near UV-visible and the other for the visible-near IR regions. The output at the end of the light guide is similar to that of our popular 300W Lambda LS xenon arc light source. The lamp module is expected to last in excess of 15,000 hours and maintain at least 50% of the original intesity at 10,000 hours.

10,000 hour expected lifetime
Low light loss throughout bulb lifetime
No high-voltage pulse
Very stable output
Compact stand-alone design, comprising bulb module, power supply and optics in a single enclosure
Output Range
300 to 700nm
Lamp Life
Approximately 15,000 hours

 

The Lambda LS is a stand-alone illuminator consisting of a xenon-arc lamp, lamp housing, cold mirror and power supply in a single enclosure. The Lambda LS is designed to be used with a liquid light guide which transmits remarkably flat, intense, illumination to the optical train of the user’s microscope or other instrumentation. The lamp’s cabinet accommodates a standard Sutter Instrument filter wheel that slides easily in and out of a slot in the light path. If desired, a second filterwheel can be mounted on the outside of the cabinet. When used with appropriate adapters, the light guide output is compatible with most common microscope systems
Output Range
Standard bulb 340nm to IR
(optional full-spectrum bulb) 200nm to IR
Lamp Type
175 or 300 Watt xenon (pre-aligned to produce collimated output)
Radiant Output
25 Watts (175W lamp) (broadband, full beam)
Lamp Life
1000 hours (Bulb warranted for 500 hours. Longer life depends on application. Expected life is 1000 hours.)
Power Consumption
175 Watts

 


The Polychrome V  is an ultra fast switching monochromator with a fully digital high precision galvanometer driven grating. Whether you do high speed ratio imaging experiments, GFP-imaging with a variety of fluorophores to be distinguished, FRET with the faintest signals to be detected, Photometry with very high time resolution or TIRF in combination with wide−, the Polychrome V with its optimized condensers for all major microscopes is your superior companion.

單光儀 ( Monochromator ) :  光纖傳導 150 W Xenon, 320 - 680 nm ( 15 nm bandwidth ), 1.4 ms wavelength switch, 可達 400 nm/ms 的波長掃描速率. 其他波長與光源強度為選配.

 

wavelength range 320 nm to 680 nm
lamp type 150 W Xenon high stability lamp
lamp lifetime 2000 h (average)
output power > 10mW at 470 nm with new lamp
monochromator scanner mounted diffraction grating
half-power bandwidth 15 nm
λ − switch speed up to 400 nm/ms
optical fiber UV/Vis quartz/quartz fiber, length 2 m or 3 m
interface voltage (−10 V to +10 V), RS232, trigger in and out
operating voltage 110 V − 120 V, 210 V − 240 V

  TILL Polychrome V Monochromator 型錄下載

 
The Oligochrome is the new high-end light source from TILL Photonics. It is an ultra fast filter switching device with a fully digital high precision galvanometer driven mirror. Whether you do high speed ratio imaging experiments, GFP-imaging, FRET or others the Oligochrome with its optimized condensers delivers outstanding brightness.
lamp lifetime 2000 h (average)
wavelength range 320 nm to 700 nm or
lamp type 150 W Xenon high stability lamp
filter 5 equally illuminated filter positions
switching speed < 3 ms
optical fiber UV/Vis quartz/quartz fiber, length 2 m
interface RS232, trigger in and out, DG-4 compatible
operating voltage 110 V − 120 V, 220 V − 240 V
TILL Oligochrome 型錄下載
 
 

脈衝式紫外光源

-system with high energy and moderate repetition rate
-up to 220 J / pulse, flash energy adjustable
-pulse length from 500 to 1000 µs at full width half maximum
-manual and external (TTL compatible) trigger, electrically isolated
-white spectrum (250-1100 nm) or filter selected spectral output
-quartz optics
-fibre optically coupled system
-rich choice of light guides available

Applications:

-Flash Photolysis
-Flash Excitation
-Illumination


MicroPoint makes possible simultaneous and precise illumination and ablation of multiple regions of interest. MicroPoint allows concurrent viewing of laser illumination or laser ablation in widefield and/or fluorescence through the microscope eyepiece or with the imaging system in real time. These capabilities minimize collateral sample damage.

Systems consist of a tunable, fiber optic pumped laser source, coupling and beam steering optics, a microscope adapter, and a selection of beam splitters and interference filters. The angular alignment of the pulsed dye laser illumination is controlled via a 2-axis joy stick. The spatial alignment of is computer controlled via galvonometer beam steering optics.  The focus in the z-direction is controlled with a knurled focus ring. A motorized optical attenuator precisely adjusts power at the sample plane.  The computer controlled MicroPoint is available with a USB or Bluetooth interface

應用領域 ( Life science )
  • Laser Ablation
  • FRAP
  • FRET
  • Photoactivation
  • Photobleaching
  • Photoswitching
  • Photoconverting
  • Cell Degeneration/Regeneration
  • Release of Caged Compounds
  • Drug Delivery
  • Thrombosis
  • Free Radical Release
  • CALI
MicroPoint 型錄下載

 

為了提供更多螢光影像應用, 如長時間活細胞影像擷取與觀察 ( Fluorescence / DIC ), 多重螢光雜染影像擷取與觀察, 螢光離子流應用. 在我們的系統整合支援下, 我們也可提供附加組配, 例如 :

附加 2 - 12 孔 超高速旋轉螢光濾鏡轉盤 ( 可選配超高速快門控制 ), 切換速率可達 30 msec. Leica DMI 6000 B 可同時加裝附加 3 組 Filter-Wheel. ( 一組穿透光, 二組反射光源 ). 應用範疇包括  FRET, Ca+2 Imaging, FISH imaging, Multi-color Fluorescence Imaging, Fluorescence / DIC imaging , 1D - 6D Multi-Dimensional Imaging , Cell Tracking, Bio-mapping, Drug screening, Time Lapse with 3D reconstruction .......... 等高階顯微鏡應用.

當你做 Uncage 實驗時, 我們特別提供專業的 Flash UV Lamp ( 及 雷射 ). 光纖傳導, 高能量高功率的 Flash UV Lamp 脈衝閃光燈源 350 J/pulse. 脈衝再現率 0.2- 8 Hz. 廣泛應用於 Caged compound, Flash Photolysis

其他螢光適用燈源 Hg 50W, Hg 100W. ( 其他功率燈源, 如 250 W 等 )
Xenon 75W, 125W ( 其他功率燈源 )
派衝式二極體燈源
標準螢光模組臂括下列 3 項 :
Epi-Fluorescence Illumination Module
Light sources ( Lamp housing, Lamp, Power supply ) - ND-Filters. ( 選配 Monochromator, Galvometer-driven switcher )
Fluorescence Filter System ( Filter cubes, Excitation Filter, Beam-Splitter, Emission Filter )
然而, 我們也必須指出, 樣品處理與染劑選擇, 也是螢光觀察技術極為重要的考量因素.
如果擬建立自動化影像擷取系統, 則可考慮濾鏡轉盤 ( Programmable Filter-Wheel ), 快門 ( Shutter ), 電控 X-Y / X-Y-Z 載物台 ( Motorized stage ).
相關連結與下載 :

Leica 顯微鏡 螢光濾鏡參考資料

數位影像系統 參考資料

Leica 顯微鏡 物鏡參考資料

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Discovery 研究級顯微鏡
美嘉儀器股份有限公司 Major Instruments Co., Ltd.
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