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Leica
DM LFS, DM LFSA
載物台固定式
( 鼻輪升降 ) - 正立顯微鏡
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| Leica
DM LFSA
是智慧型微電腦控制式的載物台固定,
鼻輪升降式
紅外光顯微鏡 (
Infra-Red Microscope ).
Leica
DM LFS
則為機械式,
或,
電控式的紅外光顯微鏡
(
Infra-Red Microscope ).
主要應用於
:
神經細胞電生理紀錄,
離子流影像紀錄,
紅外光-Patch-Clamp
紀錄, 腦組織切片紀錄,
以及共軛焦與多光子影像應用.
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Leica DM LFSA 是依據從事
Patch-Clamp
的諾貝爾獎得主與
IR-Patchclamp
的研究人員,
所合作開發的顯微鏡.
其卓越的性能與豐沛的使用效益,
提供了新一代顯微鏡的標竿. |
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微電腦可程式控制
( 配有
Leica
獨家的顯微鏡控制器
CTR-MIC
), 具有記憶學習的智慧型六孔鏡頭旋轉鼻輪,
自動升降聚焦,
自動浸水,
自動調光,
完全不會干擾碰觸記錄微電極,
完全不會產生任何震動干擾.
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專利的
"
Dip-in " 讓不同倍數的鏡頭
( 水鏡
),
都能精確的浸水,
旋轉,
再聚焦.
此種複雜精密的設計,
除了 Leica
DM LFSA 外,
沒有其他廠商有此設計.
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載物台固定,
鼻輪升降行程長達
26
mm 以上,
最適於顯微操作
(
In-Vivo / In-Vitro )
我們提供顯微鏡
X-Y,
X-Y-Z 移動光學桌.
( 包括手動 或
電控操作 )
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Leica
的 CTR-MIC
微電腦可程式顯微鏡控制器,
是未來的顯微鏡控制中心.
其可控制所有顯微鏡及未來附加的控制器
( 例如,
X-Y scanning stage, Laser Microdissection ). 使用人員只要使用控制旋鈕
(
Control Knob ) 即可操作顯微鏡,
例如鏡頭旋轉,
調焦,
調光. |
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Leica
DM LFS, DM LFSA 完全無電場干擾,
顯微鏡基座本身已將大部分的電子元件移至外接控制器,
使用人員可使用控制旋鈕
(
Control Knob ) 來操作顯微鏡,
例如鏡頭旋轉,
調焦,
調光.
Leica DM
LFSA 內建 IR:700
nm ( IR 700-850 nm ) 的 IR-濾鏡,
可同時供多光子共軛焦及紅外線
CCD 攝影機,
電生理紀錄電極等合併使用.
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專利的聚光鏡
(
Condenser ) 具有防水及排水設計. |
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專利的三眼照相攝影鏡筒,
同時可使用紫外光及紅外光應用.
而紅外光專用的雙出口攝影鏡筒,
可同時使用一般攝影機及紅外線攝影機
( 同時具有
1x/4x 輔助倍率放大功能
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Leica
新一代
U-V-I
HCX APO L
長工作距離水鏡
( Water
immersion objectives )
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| 鏡頭種類 |
適用光譜 |
工作距離 |
鏡頭開口廣角角度 |
| HCX
APO L 10x / 0.30 W |
U-V-I |
3.7
mm |
>
45 度 (
耐酸鹼耐腐蝕陶瓷琺琅
) |
| HCX
APO L 20x / 0.50 W |
U-V-I |
3.6
mm |
>
45 度 ( 耐酸鹼耐腐蝕陶瓷琺琅 ) |
| HCX
APO L 40x / 0.80 W |
U-V-I |
3.3
mm |
>
45 度 ( 耐酸鹼耐腐蝕陶瓷琺琅 ) |
| HCX
APO L 63x / 0.90 W |
U-V-I |
2.2
mm |
>
43
度 (
耐酸鹼耐腐蝕陶瓷琺琅
) |
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U-V-I
: 代表光學修正從
350
( UV ) - 可見光 (
VIS ) 到 1000
nm ( IR ), 完全修正,
完全 Parfocal,
最適於偏光,
紅外光,
螢光,
活細胞影像,
多光子影像應用. |
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| 光譜穿透效率 |
UV
( 340 nm ) |
VIS |
IR
( 1000 nm ) |
| HCX
APO L 10x/0.30 W |
78% |
95% |
85% |
| HCX
APO L 20x/0.50 W |
40% |
91% |
73% |
| HCX
APO L 40x/0.80 W |
51% |
91% |
74% |
| HCX
APO L 63x/0.90 W |
42% |
89% |
74% |
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所有 U-V-I
水鏡,
當應用於干涉對比時
(
DIC ), 僅共用一個
D1-
prism 菱鏡即可,
此為 Leica
突破性的光學設計,
近紅外光的 DIC
影像,
立體鮮明.
當使用螢光與干涉影像重疊時,
其影像解析的表現,
令人激賞. |
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相關主要配備
( 依照應用規劃組配 ) |
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光學防震桌
(
Vibration-Free Optical Table ) |
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桌上型,
光學防震桌
(
Breadboard ) |
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顯微操作系統
(
Micro-Manipulation, Micro-Injection,
Micro-Perfusion ) |
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| Narishige
/ Eppendorf / Sutter 微調與注射設備,
拉針器
(
Micropipette Puller, 研磨器
(
Micro-Grinder ), 電極處理器
(
Microforge ). |
| 美嘉儀器獨家代理美國
Sutter
知名儀器,
產品包括
Micropipette
Puller, Micro-Beveler, Filter Wheel,
Wavelength switcher, Motorized
Micromanipulator, Isolation-Free Stand,
Micro-syringe. 同時,
也代理經銷
Narishige
產品.
我們皆已累積超過
17
年的歷史. |
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可調光學桌,
可適用所有廠牌顯微鏡與及倒立顯微鏡. |
Microforge
應用於玻璃微管
(
Microelectrode ) 的燒灼製作. |
Sutter
的 P-97
與 P-87,
P-2000 拉針器. |
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電生理紀錄系統
(
Patch clamp - Electrophysiology ) |
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紅外線
CCD
攝影機 |
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我們已成功的安裝數套結合紅外光影像與電生理紀錄系統,
供 Patch-Clamp
與 Brain
slice 應用.
Hamamatsu
IR-CCD Camera 是不可或缺的影像擷取器. |
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FEATURES
Leica
DM LFSA
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BENEFITS
/ ADVANTAGES
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Patented
automatic change of objectives “Dip-in”
feature
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Specimens
are undisturbed during
objective change over
no difficult reception of water and
no loosing the focus
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Programmable four “Z” thresholds
for each objective
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individual mode of objective change
improves experiment set-up time and reduces
chance of tearing the patch
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Automatic electronic parfocalization
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Makes finding and focusing on samples
easily achieved
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More than 2 nosepice positions
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Higher flexibility
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Electronic nosepiece turret
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Fine and smooth movements, neither
mechanical shearing nor vibrations
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Multifunctional control panel
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No
touching the instrument for objective change
or lamp adjustment
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Remote focus control
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Precise
focusing without touching the microscope
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PC-download to the control panel
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Individual software interface and
fast software updates
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Power switched off after objective
change
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No disturbing electric signals during
measurement
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Microscope
Zero pixel
shift fluorescence
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Accuracy of
data for co- localization
is improved without software
corrections
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Patented
condenser draining system
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Allows easy
drainage of culture media in the event of
spills from specimen area
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2 gears
nosepiece focusing (26mm)
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Provides
extreme stability; not affected by
additional microscope components or imaging
devices onto stand
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Narrow
microscope footprint with greatest possible
free working area
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Allows easy
access for manipulator platforms and
multi-micromanipulator set-ups for
micropipette/microelectrodes
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12V 100W
lamp housing with shielded cable (3m)
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Allows
minimal electrical interference
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Observation
head with special VIS/IR tube lens
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Optimal IR
high performance imaging
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Focus stop
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Guaranties
exact focusing after objective change and no
damage of dishes or chambers
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IR
parfocalized Video Magnification Changer
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Allows
convenient method in IR to increase 1x-4x
magnification without changingobjectives
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Objectives
with narrow front lens and access angle >
45°, „U-V-I“
designation, Excellent
for POL, excellent for Fluor and excellent
for DIC even in NIR,
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Allows easy
access for micropipettes and microelectrode
probe tips, Suitable
for UV, visible, and IR imaging, Compatible
for all Leica microscopes and all its
applications in fluid media
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Leica
針對 DM
LFSA 的設計述求 |
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Patented,
fast Objective change on a turret without
readjustment of micro-electrodes or
micro-tools. Accomplished by a 90° tilted
nosepiece to swing the objectives in
the instruments longitudinal axis, with an
dip-in and parfocality algorithm.
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Fast
selection between 2 or 3 objectives possible
(in case of special set up of electrodes up to
6 different objectives)
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Minimized
vibration in comparison to a manual objective
slider
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Automatic
change of objectives without manual touching
the instrument
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Parfocality
after objective change (no long search for
focus)
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Every
objective (its specs, its movement, position
and speed) is individual programmable. PC set
up via RS232 interface to the microscope.
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Controlled
by PC or a 2 knob control unit
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Easy
to use with the 2 knob control unit
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Light
control by the same control unit
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PC-download
to the control unit
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Integrated
power supply in control box, only 1 power
supply for the entire microscope
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Inactive
electronic during measurement (no electronic
interference)
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Fast
up and down travel of objective to check
electrodes (back in focus after dip-in and
pick up the water to the objective)
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Programmable
travel of Z-movement
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(e.g.
up to 4 thresholds can be defined: Park
position, Home position and/or Focus position
and/or Dip-in position)
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Automated
back in focus after objective change or after
move into Park- or Home position
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Adaptation
of Leica Confocal Microscopy (TCS) possible.
- All
USPs (NIR-DIC, UVI-optics, Space and
stability, etc.) of the known DM LFS are still
valid
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Microscope
stand must be capable of having a precise
focusing nosepiece travel mechanism of 26mm
and of allowing suitable fixed stage
microscope attachments of manipulator
platforms and/or x-y stage for microscope
movement.
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Microscope stand must be capable of the
attachment of 2 transmitted and 2 reflected
light illumination systems that are switchable
with a manually operated mirror system.
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Microscope stand must be capable of 5
place nosepiece for transmitted light
BF/PH/DF/DIC systems.
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Microscope stand must be capable of
accepting a sealed condenser system with
provision for liquid drainage in the event of
media spillage from the specimen area.
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Microscope stand must be capable of
accepting a sealed condenser system for full
Koehler illumination for objectives 10x-100x
with an 4 place condenser turret system for
selected phase and darkfield annuli, and DIC
prisms.
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Microscope stand must be capable of
utilizing objective DIC prisms sliders with
fine vernier adjustment and must allow an open
dust-free position for normal brightfield
viewing without removal of the slider prism.
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Microscope stand must be capable of a
accepting long working distance water
immersion APO corrected objectives with
spectral transmission suitable for UV340nm
fluorescence, visible, and NIR (Near IR)
interference contrast, an acceptance angle for
micropipette or microelectrode probe tips of
greater than 45 degrees.
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Microscope stand must be capable of
accepting PL Fluotar 10x/0.30, 20x/0.50,
40x/0.70, 100x/1.30 and/or long working
distance, immersion HCX APO L U-V-I 10x/0.30W,
20x/0.50W, 40x/0.80W and 63x/0.90W with a
single DIC objective prism slider.
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Microscope stand must be capable of
accepting long working distance water
immersion HCX APO L
U-V-I objectives and all vitally
positioned objective components as
non-metallic and non-electrically conductive,
all with excellent water tension properties,
with flat front lenses, and suitable for
UV340nm, visible and IR imaging.
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Microscope stand must be capable of
accepting up to four epi-fluorescence filter
combinations and allow rapid and easy
repositioning of the order of the filter
combinations without the use of tools.
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Microscope must be capable of 0-pixel
shift technology which allows the rotation of
normally provided fluorescence filters cubes
without observing image shifts.
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Microscope must be capable of
simultaneous observation of DIC-Nomarski and
epi-fluorescence operations without
compromising the four different fluorescence
filter combinations and/or positions.
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Microscope stand must be capable of
easily switching from all transmitted light
techniques and epi-fluorescence to all
reflected light BF/DF/POL/DIC systems.
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Microscope must be capable of accepting
a standard or optional NIR corrected
observation tube with beam splitter with
100/0; 50/50; 0/100% settings for imaging.
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Microscope observation tube must be
capable of accepting an additional dual
imaging beam splitter with a dichromatic
mirror that allows simultaneous split of 100%
visual and 100% IR to two different ports.
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Microscope observation tube must be
capable of accepting an optional IR
parfocalized magnification 1x-4x changer
system.
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Microscope must be capable of accepting
ergonomic tilting observation tubes and
tilting observation phototubes.
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Microscope must be capable of accepting
optional observation tubes capable of
accepting macro-viewing and slide overlay
devices suitable for overlaying macro,
drawing, or 35mm slide images onto the
microscope imaging system.
- Microscope
must be capable of accepting 35mm
photo-micrographic camera system with
illuminated focusing reticule in the binocular
phototube with spot measurement down to 0.1
degree.
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一些主要應用 |
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Neurophysiology
Electrophysiology
Neurobiology
Neuroscience
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Thick
brain slice (200mm)
patch clamp recording
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Patch
clamp recording ( IR-Patch setup )
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Microelectrode
recording
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Whole
cell recording
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Fluorescence
uncaging
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Fluorescence
microscopy
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Intracellular
pH recording
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Oocyte
patch clamp
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Two
electrode voltage clamping
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Near
IR interference contrast
- Confocal
& Multi-Photon microscopy
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Cell
Physiology |
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Indo/Fura-2/Ca++
rationing systems
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Ca++,
Na+, K+ ion channels
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Intra
vital microscopy
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Adherent
whole cell recording (tissue culture)
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Ion
channel and transport studies
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Cell
membrane permeability studies
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Protein
transport studies
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Drug
sensitivity studies
- Intracellular
perfusion
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Cell
biology |
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Green
Fluorescence Protein (GFP)
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Time
lapse cell growth recordings
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FRAP
(Fluorescence recovery after photobleaching
- Adherent
whole cell recording (tissue culture)
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Micro
Injection and Micro Manipulation in Cell Biology |
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