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其他相關設備
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Laser
Illumination & Ablation System
........可適用於所有廠牌顯微鏡 |
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以雷射作為光源, 在樣品上 ( 無論是在明視野或螢光
), 以可調的雷射光點, 作為指定局部的顯微照明 ( micro illumination ) .
可應用為顯微照明及一種顯微的消融工具 ( micro ablation ). 雷射光點及強度皆為可調. |
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Ablation /
MicroPoint Laser Illumination System used for ablation of
muscle precursor cells in C. elegans
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Time course
of development of a single wild-type embryo in which
the muscle precursor cells, Cap and Cpp, were ablated
(dorsal view). Despite the absence of ~1/3 of the body
muscle (primarily posterior and dorsally positioned
cells), intercalation occurs normally. Intercalating
dorsal cells in A, B, C are indicated by arrows.
A) Start of
intercalation.
B) Intercalation partially complete.
C) Intercalation complete.
D) Interior focal plane; arrowheads indicate
undifferentiated ablated material. Times indicate
amount of time elapsed from the beginning
intercalation. Scale bar = 10 mm.
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Cell
Regeneration /
MicroPoint Laser Illumination System used for cell
regeneration degeneration |
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First graphic
shows ablation of spiral ganglion neuron. Second
graphic shows related apoptotic death of its sensory
inner hair cell in 6 day old organotypic culture of
the newborn mouse cochlea 18 hours after single pulse
laser beam injury. |
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FRAP |
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Yeast cells
are labeled with Nuf2p::GFP to mark the spindle pole
bodies and Ase1p::GFP in the mitotic spindle mid-zone.
They are observed in DIC and fluorescence microscopy.
A) Metaphase spindle before elongation.
B) Telophase spindle at the end of Anaphase.
Ase1p::GFP remains localized to the spindle
mid-zone throughout mitosis.
C) Ase1p::GFP photobleached on the metaphase spindle.
D) Ase1p::GFP completely recovers within 20 minutes as
the spindle elongate. |
Haemostasis
/
MicroPoint Laser Illumination System used to induce
Thrombosis
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Platelet
thrombosis formation followed laser induced
endothelical injury. FITC-anti-rabbit IgG and rabbit
anti-CD41 were infused into the mouse via a jugular
catheter. CD41 is located on platelets and FITC is
associated with most platelets in the mouse
circulation. Upon laser-induced endothelial injury of
the arteriole with the MicroPoint Laser System,
platelets become associated with the injured
endothelial surface. Laser induced injury targeting
the larger veins is shown near the base of the ear.
A) Before
injury.
B) 30 seconds after injury.
C) 1 minute after injury.
D) 2 minutes after injury.
E) Vasculature of mouse ear |
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Heat Shock
Gene Promoter /
MicroPoint Laser Illumination System used for Heat
Shock Gene Promoters in zebrafish
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Heat Shock
Gene Promoter In Zebra Fish Individual cells in hsp
70-egfp transgenic embryos can be induced to express
EGFP by targeting them with a MicroPoint Laser System.
All panels are side views with anterior to the left
and dorsal up. (A-C) Images of the developing ear in a
living transgenic embryo 1 day after laser induction
of an individual cell in the otocyst at 16 hpf (hours
post fertilization), demonstrating that a single cell
can be specifically induced and that the targeted cell
appears undamaged
A) DIC image.
B) Fluorescence image of the same field as A.
C) Superposition of A and B. Arrows indicate targeted
cell.
D) Fluorescence image of a laser targeted CiD neuron
in the spinal cord of a 33 hpf living transgenic
embryo. The neuron was targeted at 15 hpf, before axon
outgrowth, and has subsequently extended an axon
several hundred microns down the spinal cord. The axon
has branched and each branch is tipped with growth
cones (arrows). The cell body is at level of somite 4
and growth cones at somite 9.
E) A DIC image of the tailbud at the stage tailbud
cells were laser targeted at 16 hpf. Individual
progenitor cells in the region posterior to Kupffer¡¦s
vesicle (k) were induced.
F, G) DIC(F)and fluorescence of the same 36 hpf embryo
in which one tailbud progenitor cell had been induced
at 16 hpf. Arrows indicate the same region of both
images. In this embryo, 6 EGFP-expressing somite cells
were present in the posterior tail somites at 36
hpf. Scale bars, A-D, F, G, 20:m.
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Release of Caged
Compounds /
MicroPoint Laser
Illumination System used to release caged compounds in
zebrafish
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A) Labeled
single cell of a germ-ring stage at 6 hpf zebrafish
embryo (5 X magnification).
B) Labeled single cell of 40% epiboly 5 hpf zebrafish
embryo.
C) Labeled .Tier one marginal cells of a 40% epiboly
at 5 hpf zebrafish embryo.
D) Single (or two) labeled notochord cells of a
5-somite stage at 12 hpf zebrafish embryo (40X
magnification). |
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模組化的顯微鏡顯微局部螢光激發用雷射光源,
雷射消融裝置.
- MicroPoint Laser Illumination and
Ablation Systems
- Mosaic Digital Illumination Systems
MicroPoint
makes possible laser illumination and ablation of near
diffraction limited geometries. MicroPoint allows
simultaneous viewing of the laser application in
widefield and/or fluorescence through the microscope
eyepieces or with the imaging system in real time.
Systems consist of a tunable, fiber optic pumped
laser source, coupling and beam steering optics,
microscope adapter, and a selection of beam splitters
and interference filters. Angular and spatial
alignment of the illumination at the sample target is
manually controlled via a 2-axis joy stick. Focus in
the z-direction is manually controlled with a knurled
focus ring. The MicroPoint manual systems use a
motorized or manually driven optical attenuator to
adjust spot size and power. Units may be upgraded in
the field to include computer control and additional
wavel
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生醫應用 |
Life Sciences
- Fluorescence Recovery After Photobleaching
(FRAP)
- Fluorescence Energy Resonant Transfer (FRET)
- Photoactivation
- Photobleaching
- Photoswitching
- Photoconverting
- Total Internal Reflection Fluorescence (TIRF)
- Cell Regeneration/Degeneration
- Release of Caged Compounds
- Drug Delivery
- Thrombosis
- Free Radical Release
- Chromophore Assisted Laser Inactivation (CALI)
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工業應用 |
Semiconductor Processing
- Laser Ablation
- Circuit Isolation
- Marking IC's
- Marking Hard DIsc & Media
- Flip Chip Navigation
- LCD Repair
- Micromachining
- Navigation
- Removal of Passivation
- Removal of Photoresist
- Semiconductor Failure Analysis
- Probe Stations
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儀器分類 |
三類產品組配供選擇 ( 訂購者,
請告知顯微鏡廠牌及機型, 包括應用 )
Three Mosaic and MicroPoint systems are available
to most effectively accommodate application
requirements and budget. Manual MicroPoint systems may
be upgraded to Computer Controlled MicroPoint systems.
Please contact
Photonics Instruments for assistance in selecting
a MicroPoint system.
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Mosaic Digital Illumination |
MicroPoint Computer Controlled Illumination and
Ablation |
MicroPoint Manual Illumination and Ablation |
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Technology |
Digital micromirror device (DMD) |
Galvanometer beam steering system |
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Control |
Computer controlled with software
supplied by Photonic Instruments or its partners |
2 axis joy stick and manual z focus |
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Mask flexibility - shape,
resolvable size, complexity |
Unlimited - multiple, diffraction,
translucent, infintely complex shapes |
Near diffraction limited multiple spots |
Near diffraction limited single spots |
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Illumination of region of
interest |
Yes, most complex geometries with highest
precision |
Yes |
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Simultaneous illumination of
multiple regions of interest |
Yes, zero delta time between regions |
No |
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Speed and throughput |
Highest, zero delta time imaging of multiple
regions |
Moderate |
Lower |
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Molecularly selective
ablation and illumination with one laser system |
No* |
Yes |
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Low cost wavelength
modularity |
Cost effectively add wavelengths with Mercury
lamp in field and cw laser in factory |
Most cost effectively add
wavelengths in field with tunable laser |
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Compatible with point/slit
scan confocal microscopy platforms |
Yes, via epi-port, C-mount and broad offering
of adapters |
Yes, via multiple ports and broad
offering of adapters |
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Compatible with spinning
disk confocal microscopy platforms |
Yes, via epi-port, C-mount and broad offering
of adapters |
Yes, via multiple ports and broad
offering of adapters |
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Compatible with wide field
microscopy platforms |
Yes, via epi-port, C-mount and broad offering
of adapters |
Yes, via multiple ports and broad
offering of adapters |
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Ruggedness & reliability |
Highest, based on semiconductor device |
High |
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Maintenance |
Lowest, semiconductor device, modular system
independent of microscope |
Low, modular system independent of
microscope |
* Mosaic Digital Illumination System may be
multiplexed with a Computer Controlled MicroPoint
system for applications requiring both ablation and
zero delta time fluorescence excitation.
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與原廠連結的動態電影檔 ( 客戶使用
Micropoint & Mosic 應用 )
Ablation
Micro-surgical cell membrane cut
Cell wounding
FRAP
Simultaneous FRAP of complex shapes
High speed FRAP kinetics
FRAP in C.Elegans
Photoactivation
Diffraction limited photoactivation with TIRF
GFP photoactivation kinetics
Simultaneous photoactivation of complex shapes
GAG-GFP photoactivation for particle tracking
Compartmentalized dynamics
Photoactivation in C.elegans
Uncaging
Calcium release
Thrombosis
Laser induced thrombosis
The Mosaic’s
proprietary digital illumination technology is a
robust and flexible platform amenable for
OEMs to develop other fluorescence imaging
applications in the life sciences such as High Content
Screening, in-vivo and ex-vivo tissue and small animal
imaging, biosensors, spectroscopy and microendoscopy.
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