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FCS
2
Fluorescence
Correlation Spectroscopy
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Leica 的 FCS
2 系統, 是全世界最好的
Spectral Confocal FCS
設計 !
結合 Confocal imaging 與
FCS 量測分析 !
FCS 2 技術適用於分析活細胞的分子間交互機制,
傳送機制. 可做到單分子的定量濃度分析
及 擴散速率的分析.
建議採用 Leica TCS SP2
AOBS 共軛焦系統, 外接
FCS 2 模組與控制分析系統.
您可洽購 FCS
2 完整系統 (
TCS SP2 AOBS with FCS 2 ) 或 FCS
2 升級模組
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應用範例 :
• Molecular Dynamics
• Kinetic rate constants
• Antibody-antigen interactions
• Ligand-receptor interactions
• Nucleic acid hybridization
• Molecular aggregation, polydispersity, and
molecular weights
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| Leica
TCS SP2 AOBS with FCS 2 system 圖示 |
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| Application
software for one-channel and two-channel data
acquisition, either in time mode or photon mode. It
features a real-time display of the auto-correlation
and cross-correlation functions (as in the plot
above) and a sequence of multiple data acquisition
files can be acquired, displayed and stored
automatically |
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The
software enables the user to select the size,
resolution and type (steady-state/lifetime) of
image to be acquired. Additionally, the
residence time at each pixel is user
selectable. Image of the same XY region can be
acquired at different z-axis positions. The
user can also select a region within the
original image and start a new acquisition of
the zoomed area. Upon acquisition, the image
can be processed using the filters included in
Vista. Images can be exported in several
formats (bmp, gif, jpeg, tiff, png). |
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相關性能 :
Computer
controlled selection of the confocal pinhole size
Computer controlled alignment of the confocal
pinhole and optics
Computer controlled positioning of the objective
(z-axis)
Computer-controlled XYZ microscope stage
Single-photon or multi-photon excitation
One channel or dual-channel data acquisition
Avalanche photodiodes or cooled photo-multiplier
tubes detectors
Measurement
( 量測 )
Confocal
fluorescence images
Confocal fluorescence lifetime images
Fluorescence Correlation Spectroscopy (single
channel and cross-correlation)
Photon Counting Histogram (PCH)
Polarization measurements
Particle tracking
When using autocorrelation and
cross-correlation functions:
- Diffusion coefficient
- Diffusion time
- Concentration
- Triplet state decay time constant
- Triplet function
- Flow rate
- Size of excitation volume
- Number of molecules
When using photon counting histogram (PCH):
- Number of molecules
- Molecular brightness
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| Leica
FCS 2 系統,
提供獨立一組電腦工作站.
有關詳細資料與規劃,
請洽本公司技術專員. |
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