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FLIM
Fluorescence
Lifetime Imaging Microscopy
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Leica FLIM 系統,
可區分 : D
FLIM 2 ( 採用 405 nm 脈衝雷射
- Pulsed Diode Laser ) MP
FLIM 2 ( 採用 多光子飛秒超快雷射 -
Multi-Photon system, femto / pico Ti:sapphire )
建議採用 Leica TCS SP2
AOBS 共軛焦系統, 外接
FLIM 模組與控制分析系統.
您可洽購 FLIM 完整系統 (
TCS SP2 AOBS with FLIM ) 或 FLIM 升級模組
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| FLIM
is a technique to measure ion concentrations,
intracellular signal transduction, FRET
( Fluorescence
Resonant Energy Transfer ), membrane potential
and more using time-correlated single photon
counting technology. Striking advantages of this
method are its insensitivity to shading, to
photo-bleaching, concentration fluctuations of the
fluorochrom itself, to excitation intensity, and to
light source noise.
Fluorescence
Lifetime Imaging (FLIM) has become a new powerful
method to investigate molecular interactions,
metabolic reactions and energy transfer in cells and
subcellular structures. These effects cause changes
in the fluorescence quantum efficiency and thus in
the fluorescence lifetime. Since the fluorescence
lifetime does not depend on the unknown dye
concentration it is a direct measure for the quantum
efficiency. It therefore gives a more direct access
to the investigated effects than the fluorescence
intensity. Furthermore, the fluorescence lifetime
can be used to separate the fluorescence of
different luminophores in the cells if the
components cannot be distinguished by their
fluorescence spectra.
FLIM
相關技術資料 (
PDF ) (
技術,
原理,
應用,
系統 )
FLIM
相關技術資料 (
TCSSP Confocal Laser Scanning Microscopy : 技術,
原理,
應用,
系統升級)
FLIM
應用範例 :
- Determination
of local environment changes with the
same probe.
- Discriminate between similar
fluorophores e.g. GFP and YFP for FRET studies
- Discriminate between RNA and
DNA
- Ideal for discriminating
between probes and autofluorescence.
- Label-free imaging of live
samples (e.g. diagnostics of tumor tissue.)
- Complementary to spectral
discrimination.
- Measurement of
ion concentrations.
- Intracellular
signal transduction.
-
Membrane
potential.
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| D
FLIM 2 |
MP
FLIM 2 |
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D FLIM 2 extension
uses a pulsed 405 nm diode laser as excitation
source and time reference. The detection system is
coupled to the external port of the system, not
restricting the use of the basic instrument for
confocal imaging.
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The
MP FLIM 2
extension uses a multiphoton laser as excitation
source and time reference providing all the
advantages of multiphoton excitation for FLIM
analysis, like deep tissue penetration, less
autofluorescence, and less photobleaching outside
the focus.
The MP FLIM 2 detection system is coupled to
the external port of the system, not restricting the
use of the basic instrument for confocal imaging.
It is available as upgrade for Leica TCS SP2 AOBS
systems.
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有意升級擴充安裝 FLIM
( D-FLIM 2 或 MP-FLIM
2 ) 系統者,
其掃描器於訂購時,
都必須事先訂購 "
External Port ". 請洽本公司專業工程師.
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| Leica
TCS SP2 AOBS with FLIM system 圖示 |
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| Leica
FLIM 系統,
提供獨立一組電腦工作站.
有關詳細資料與規劃,
請洽本公司技術專員. |
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