徠卡顯微鏡系統

雷射掃描共軛焦顯微鏡

半導體及工業用顯微鏡

顯微數位影像系統

細胞基因影像分析系統

了解 Leica 在 Super-Resolution 的解決方案與領導趨勢 ..

多一點了解 Leica TCS SP5 的卓越, 與其他產品的差異性在哪裡 ?

全世界第一套光譜式雷射掃共軛焦實體 (3D)螢光顯微鏡系統 The World‘s First Super Zoom Confocal!

From Gene to Cell - from Cell to Embryo

Leica 創新技術, 推出第一套 TCS LSI 雷射掃共軛焦實體螢光顯微鏡系統 ( LSI : Large Scale Imaging ). 高倍率的活體螢光影像擷取, 立體螢光影像擷取, 長時間立體螢光體像擷取, 影像量測. 都進入雷射掃共軛焦實體螢光顯微鏡技術的時代了. 您可以從細胞做到胚胎, 活體影像.

Leica TCS LSI 採用最精密的雷射掃共軛焦掃描器 ( TCS ), 光譜式偵測, 採用固態雷射 405, 488, 532, 635 nm, AOTF 連續調控. 架構在 Leica Z16 APO/A 或 Z6 APO/A 顯微鏡上, 配合高速 Galvanometer-driven Z-stage, 總縱深 Z-軸行程可達 1600 μm.

共軛焦專屬物鏡 : PLAN-APO 1.0x, 2x, 5x, 10x, 40x, 63x.

長時間影擷取應用可搭配全系列的溫控及二氧化碳培養箱.

光譜式共軛焦實體顯微鏡系統 ( 配置 Leica Z16 APO A Zoom Macroscope )

Confocal technology ( with unique Leica Macroscope )

‧ Highest resolution,‧ True confocal point scanner

‧ Spectral detector

‧ Solid state laser, ‧ 405, 488, 532, 635 nm excitation, ‧ AOTF controlled

‧ Fully automated,

選配溫控二氧化碳活細胞培養箱, 電控載物台. 可應用於多孔槽 ( Multiwell ) 的活細胞 / 活體影像擷取., 長時間影像擷取.

導覽式軟體, 簡易使用, 應用軟體齊全.

Leica TCS LSI 可以實體變焦, 擁有最常的工作距離與景深. 除了 Leica Z16 APO A 的 16:1變焦比( 連續被率調整 0,57x to 9,2x ) 外, 也可以搭配顯微鏡物鏡 ( 1x, 2x, 5x, 10x, 40x, 63x )

Mouse embryo

Nuclear and neurofilament staining of mouse embryo (10.5 days post coitum)., Courtesy: ICI, Imaging Center of IGBMC, Strasbourg, France

創新的設計, 造就寬廣的應用領域實現共軛焦顯微鏡的另一效益.

Highest Flexibility (Macro and Micro: New dimensions for largest specimen )

‧ Dynamic confocal imaging

‧ Spectral band tuning

‧ 0 – 100% AOTF laser control

‧ Variable pinhole

‧ Variable magnification

‧ Motorized z-zooms

‧ Full objective band from 1x to 63x

‧ FOV max: 16 mm

‧ WD max: 97 mm

‧ Z-range max: 150 mm

Drosophila melanogaster, head of larvae. Head with nerve system and photoreceptors, Red: Cy3, Green: Alexa 488.

從班馬魚的成長做活體共軛焦影像擷取 ( from Macro to Micro )

Zebra fish. Danio Rerio. Red: Cy3, z-disk staining. Blue: DAPI, nucleoli.

Maximum projection ( Zebra fish. )

10 steps, Step size: 6.36 µm, Depth size: 57.2 µm

Maximum projection ( Zebra fish. )

20 steps, Step size: 3.88 µm, Depth size: 73.7 µm

Mouse transgenic embryo

Mus musculus. E10.5 mouse transgenic embryo: EpaxialMyf5 eGFP; immuno-stained for GFP-Alexa 488; embryonic muscle fibers and heart stained with Desmin-Cy3. Size from top to bottom: 3.5 mm. Top: RGB-channels. Courtesy of: Aurelie Jory, Cellules Souches et Developpement, Institut Pasteur, Paris, France


Zebra fish development Danio Rerio. Embryo, T=0h, 6h, 11h, 17h. Bodipy TR. Courtesy of: Jabier Gallego Llamas, Team P. Dolle, Imaging centre of IGBMC, IGBMC, Illkirch, France.	Drosophila melanogaster, nerve system development. Larvae Blue: DAPI, nucleoli, Green: Alexa 488, CNS.

美嘉公司的共軛焦影像技術團隊擁有數名博士及碩士畢業的高級技術專員, 並有長期在實驗室的工作經驗, 同時也經過數年的 LEICA 原廠培訓, 可以提供客戶優質的影像技術支援與實驗指導., 協助您各種的共軛焦影像應用.

詳細的規格與組配, 可洽詢本公司技術專員. ( 歡迎預約現場展示性能與儀器示範 )

請下載檔案 ..

Downloads Leica TCS LSI

Brochures

Leica_TCS_LSI-Brochure.EN	(PDF, 1.86 MB, 22. Jan 2008)
Leica_TCS_LSI-Brochure_Technical_Data.EN	(PDF, 417.40 K, 21. Jan 2008)
Leica_TCS_LSI_Scanhead-Flyer.EN	(PDF, 709.07 K, 05. Oct 2007)

Newsletters

microscienceimaging_5	(PDF, 699.21 K, 20. Jul 2007)
microscienceimaging_6	(PDF, 368.51 K, 20. Nov 2007)
reSOLUTION_Research_Neuroscience_09	(PDF, 3.07 MB, 17. Sep 2009)
reSolution_Research_News_No6_March2009	(PDF, 2.03 MB, 19. Mar 2009)

Leica TCS SP5 II 目錄 ( PDF, 3042 KB )

Leica TCS SP5 X 目錄 ( PDF, 578 KB )

Leica TCS SP5 II Technical Datasheet ( PDF, 381 KB )

Leica TCS SMD series 目錄 ( PDF, 3768 KB )

其他產品詳細資料, 請點選下列圖示.

Leica TCS STED	Leica TCS STED CW	Leica TCS CARS
Integrates Super Resolution STED with the Broadband Confocal Platform TCS SP5 Leica TCS STED. With the award-winning invention of STED technology, a new chapter in fluorescence microscopy began	The Fast Track to Superresolution !!! The new Leica TCS STED CW is the most uncomplex way to nanoscopy for research. The system resolves structures smaller than 80 nm – with purely optical methods............	Coherent anti-Stokes Raman scattering (CARS) microscopy overcomes a powerful tool for three-dimensional, label-free imaging of chemical and biological samples in vitro and in vivo.


Leica TCS SP5 II	Leica TCS SP5 X	Leica TCS SP5 MP
The Only Broadband Confocal Leica TCS SP5 II, The Leica TCS SP5 II Confocal covers a broad range of requirements in confocal and multiphoton imaging - with the full array of scan speeds at highest resolution ..........	Tune into any Excitation with Supercontinuum Confocal Leica TCS SP5 X. With the Leica TCS SP5 X Supercontinuum Confocal researchers can tune excitation wavelengths to perfectly match fluorophores, turn down laser power, and thereby increase viability for longer live cell experiments ......	Deep Imaging at Video Rate Leica TCS SP5 MP...........


Leica TCS SMD FCS	Leica TCS SMD FLIM	Leica TCS SMD FLCS
The system acquires and analyzes FCS and FCCS (Fluorescence Cross-Correlation Spectroscopy) data. Both methods focus on quantitative analysis of transport and binding processes, in vitro as well as in vivo	It is a dedicated system for FLIM measurements. These measurements reveal information about the direct neighborhood of molecules on the nanometer scale	Fluorescence Lifetime Correlation Spectroscopy. This single molecule detection system ensures the highest flexibility in biophysics by conveniently combining FCS (Fluorescence Correlation Spectroscopy) and FLIM (Fluorescence Lifetime Imaging) in one system................


Leica HCS A	Leica TCS SPE II	Leica TCS LSI
High Content Screening Automation. The combination of high-resolution true confocal point scanning technology with the ingenious LAS AF MATRIX M3 automation software for the first time offers high content screening on research microscopes.	The Leica TCS SPE confocal is a true point-scanning, spectral system at an affordable price for fluorescence imaging of live or fixed cells ...........	Capture Intra-vital Adult and Embryo Images from Macro to Micro with Leica TCS LSI. The first super zoom confocal that offers high resolution plus a large 16mm field of view for in vivo imaging


VT infinite 3 Pinhole array Confocal Scanner	Leica Structured Illumination Microscope	Leica 4Pi
Next-generation multi-point confocal scanner. Offers low photo-bleaching with high-speed image acquisition .......	Structured Illumination solution allows image contrast improvement, image blur removal and optical sectioning in fluorescence applications	A innovative solution for Super-Resolution .......

相關資料 ( Information )

	雷射掃描共軛焦顯微鏡技術概論與應用介紹 ( CLSM Technology & Application )
	Leica TCS 光譜式共軛焦顯微鏡的核心技術介紹 ( AOTF, AOBS, Spectral Detector )
	Leica TCS 光譜式共軛焦顯微鏡的雷射光源 ( Laser system ) 介紹
	Leica TCS 光譜式共軛焦顯微鏡的物鏡 ( Objectives ) 介紹
	Leica TCS 光譜式共軛焦顯微鏡的應用軟體 ( Application Software ) 介紹
	使用於共軛焦顯微鏡上的活細胞培養設備 ( Micro-incubation )