Precise analysis of particle transport and molecular
binding
唯一結合 FLIM 與 FCS 技術
引領"單分子偵測"的螢光顯微鏡技術進入細胞分子動力學研究與定量探索分析.
Advantages of gated FCS and FLCS
Get lifetime information and
diffusion parameter simultaneously
Remove infl uence of parasitic
signals (scattering, detector after-pulsing,
autofluorescence)
Cross-correlation studies with one
detector reduce chromatic artifacts
Applications of gated FCS and FLCS
Differentiate micro-environments in
one FCS measurement
Low concentration measurements in
sub-nanomolar range
Quantitative analysis in samples
with low signal and high background noise
Binding studies of spectrally
similar dyes using cross-correlation
FCCS
–
molecular interactions
FLIM
–
nano-environment
FLIM-FRET –
molecular distances
FLCS –
molecular dynamics in difficult samples
FLCS
的運作範例
Essentially, FCS can be performed with a
continuous-wave laser, but using pulsed lasers allows even
more sophisticated analysis possibilities, such as
time-gated FCS or Fluorescence Lifetime FCS (FLCS). Both
methods make use of the additional information obtained by
the simultaneous measurement of the fluorescence lifetime.
A time-gate, for example, selects for analysis of only
those photons that can be clearly attributed to the
fluorescence decay. Suppression of scattered light (Rayleigh,
Raman) from the detected signal allows an accurate
measurement of concentration. In FLCS each photon is
statistically weighted as to how much it contributes to
the FCS data analysis. It can be used to correct for
detector artifacts or scattering contributions. It even
allows simultaneous monitoring of two dyes with completely
overlapping emission spectra and with very similar
diffusion coefficients by using only a single detector.
Removal of detector artifacts by
lifetime filtering (FLCS)
With the award-winning invention of
STED
technology, a new chapter in fluorescence microscopy
began
The
Fast Track to Superresolution !!!
The new
Leica
TCS
STED
CW is the
most uncomplex way to nanoscopy for research. The
system resolves structures smaller than 80 nm – with
purely optical methods............
The
Only Broadband Confocal Leica
TCS
SP5 II, The Leica
TCS
SP5 II Confocal covers a broad range of requirements
in confocal and multiphoton imaging - with the full
array of scan speeds at highest resolution ..........
Tune into any Excitation with Supercontinuum Confocal
Leica
TCS
SP5 X. With the Leica
TCS
SP5 X Supercontinuum Confocal researchers can tune
excitation wavelengths to perfectly match fluorophores,
turn down laser power, and thereby increase viability
for longer live cell experiments ......
It is a dedicated system for
FLIM measurements. These measurements reveal
information about the direct neighborhood of molecules
on the nanometer scale
High Content Screening Automation. The combination of
high-resolution true confocal point
scanning technology with the ingenious
LAS
AF MATRIX
M3 automation software for the first time offers high
content screening on research microscopes.
The
Leica TCS
SPE confocal is a true point-scanning, spectral system
at an affordable price for fluorescence imaging of
live or fixed cells ...........
相關資料 ( Information )
