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雷射掃描共軛焦顯微鏡
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Leica TCS SP5

雷射掃描共軛焦光譜顯微鏡系統

組配參考

Microscope Upright Microscope - 正立顯微鏡, 微電腦全自動控制.
Leica DM 5000 CS
Leica DM 6000 CS
Inverted Microscope - 倒立顯微鏡, 微電腦全自動控制.
Leica DMI 6000 CS
Leica DMI 6000 CS Bottom- Port

* Leica TCS SP5 僅可使用於 Leica DM 或 DMI 顯微鏡上.

Optional Microscope components ( 主要選配顯微鏡模組 )
Microscope Stage ( for DMI 6000 CS Inverted Microscope only )
Motorized 3-Plate Scanning Stage - 電控載物台
Manual 3-Plate stage - 手控式載物台
Motorized Scanning XY Stage - 電控載物台
Microscope Climate Chamber System
Thermal Controlled Climate Chamber - 溫控箱 ( 大, 中, 小 )
CO2 controller ( optional ), complete set. - 二氧化碳氣體控制, 微電腦控制.
Perfusion- Imaging Chamber, complete set. - 微型培養槽.
Contrast options
Phase contrast ( Dark Field ) - 相位差 / 暗視野

DIC ( Polarization ) - 干涉對比 / 偏光
Dott contrast - Dott 立體呈相對比
BF / Fluorescence ( 標準配備 ) - 明視野 / 螢光
Digital CCD Imaging System
Cooled CCD Camera ( 冷卻式數位攝影機 )
Imaging software ( Wide-field imaging workstation ) - 影像應用軟體
Microscope automation components - 顯微鏡自動化控制相關模組.
Objectives

Microscope Objectives
Leica PL-Fluotar 共軛焦專用鏡頭
HC PL FLUOTAR 5x/0.15
HC PL FLUOTAR 10x/0.30
HC PL FLUOTAR10x/0.30 Ph1
HC PL FLUOTAR 20x/0.50
HC PL FLUOTAR 20x/0.50 Ph2
HCX PL FL 40x/0,75 / 0,17 D 0,40
HCX PL FL 40x/0,75 Ph2
PL FLUOTAR 40x/1.00 Oil Ph3
PL FLUOTAR 40x/1.00-0.50 Oil
HC PL FLUOTAR 50x/0.80
HC PL FLUOTAR 100x/0.90
HCX PL FL 100x/1.30-0,60 Oil 0.17/D
HCX PL FL 100x/1.30 Oil PH 3 0,17D
HCX PL FL 100x/1.30 Oil 0.17/D
Leica PL-APO ( CS 級 ) 共軛焦專用鏡頭
HCX APO L 10x/0.30 W
HC PL APO 10x/0.40 CS
HC PL APO 10x/0.40 lmm CS
HCX APO L 20x/0.50 W  U-V-I -/D 3.5
HC PL APO 20x/0.70 CS
HCX PL APO 20x/0.70 imm corr Lbd. BL
HC PL APO 20x/0.70 lmm Corr CS
HC PLAN APO 20x/0.70 PH2 0.17/C 0.59
HCX PLAN APO 40x/0,75 PH2 0.17
HCX PL APO 40x/0,75 U-V-I 0.17
HCX APO L 40x/0.80 W UVI
HCX PLAN APO 40x/0.85 corr
HCX PL APO 40x/0.85 CORR CS
HCX PL APO 40x/1.25 OIL PH3 CS
HCX PL APO 40x/1.25-0.75 Oil CS
HCX APO L 63x/0.90 W
HCX PL APO 63x/1.20 w corr Lbd. BL
HCX PL APO 63x/1.20 W Corr/0.17CS
HCX PL APO 63x/1,30 Glyc Corr 37°C
HCX PL APO 63x/1.30 GLYC CORR CS 21
HCX PLAPO 63x/1.4-0.6 oil CS
HCX PL APO 63x/1.40-0.60 Oil,Lbd.BL
HCX PL APO 63x/1.40 Oil PH3 CS
HCX  APO 100x/1.3 Oil U-V-I
PL APO 100x/1.40 Oil Ph3 CS
PL APO 100x/1.40-0.70 Oil CS
TCS SP5 Basic Module
TCS SP5 Basic Module with AOBS system
TCS SP5 Basic Module with Dichroic system
Optional External Port ( for FCS, FLIM, Spectral Detection Application )
　
TCS SP5 Scanning Module
Conventional Scanner ( C )

Resonant Scanner ( R )
Tandem Scanner ( T ) - ( C + R scanner )
TCS SP5 SP-detectors ( Spectral PMT )
1 channel Spectral Detection PMT
2 channel Spectral Detection PMT
3 channel Spectral Detection PMT
4 channel Spectral Detection PMT
5 channel Spectral Detection PMT
1 channel Spectral Detection FLIM PMT
2 channel Spectral Detection FLIM PMT
TCS SP5 Bright Field Detection PMT ( for Microscope )
BF-Detection PMT ( for Upright Microscope )
BF-Detection PMT ( for Inverted Microscope )
Super-Z Galvanometer Stage
Super-Z Galvanometer Stage, DM Upright Microscope ( optional Insert stage )
Super-Z Galvanometer Stage, DMI Inverted Microscope ( optional Insert stage )
Microscope Table
Microscope Table, active. ( Isolation-Free optional Table with Air-compressor )
Microscope Table, Passive 
TCS SP5 workstation with Leica LAS AF ( SP5 system software ). Included optional Workstation Table.
SP5 Workstation with 2-LCD Monitor, Windows XP, Computer Workstation Table,  Leica LAS AF system software ( TCS SP5 system software )
TCS SP5 control Panel SP5 programmable Control Panel, LCD display.
Optional application software modules
LAS AF SP5 Live Data Mode
LAS AF SP5 3D Visualisation
LAS AF SP5 Colocalisation
LAS AF SP5 Deconvolution
LAS AF SP5 MicroLab
LAS AF SP5 MotionSpy
LAS AF SP5 Macro Developer
雷射光源選配 ( UV-VIS-IR )

組配參考

雷射光源

Laser system
Laser Ports ( for SP5 Scanner - Basic Module )
Laser Port UV/405nm
IR Imaging Port inverted (MFP)
IR Imaging Port upright (MFP)
UV Imaging Port Ar only
Laser Port 405nm pulsed
雷射光源 ( 可見光雷射 )
所有可見光雷射皆光纖傳導, 8-channels AOTF 調控. Ar 雷射可選配噪音消除設備.
雷射代號 R O Y G B V
雷射組合 A 　 　 　 　 　 　
雷射組合 B 　 　 　 　 　 　
雷射組合 C 　 　 　 　 　 　
雷射組合 D 　 　 　 　 　 　
雷射組合 E 　 　 　 　 　 　
雷射組合 F 　 　 　 　 　 　
雷射光源 ( 紫外光雷射 )
Leica TCS SP5 可以同時使用 Ar-UV 351 nm 雷射 及 DPSS 405 nm near UV 雷射.

Diode UV 雷射, 405 nm, 50 mW
Ar-UV 雷射, 351, 364 nm, 50 mW
FLIM 適用的脈衝雷射, Diode 3 mW, 405 nm Pulsed UV laser.
UV/405 Laser Attenuation Module ( optional ) 
UV/405 Attenuation Filter Wheel Module
UV/405 AOTF Module
UV Correlation Pinhole Lens ( optional )
UV Optics 01
UV Optics 02
UV Optics 03
UV Optics 05
UV Optics 06
UV Optics 07
UV Optics 08
UV Optics 09
UV Optics 10
UV Optics 12
雷射光源 ( 紅外光雷射 - 多光子雷射系統 )
Mai Tai HP Specifications
Output Characteristics1 
Mai Tai HP Performance  700 nm  800 nm  920 nm  1020 nm 
Average Power2  >1 W  >2.5 W  >1 W  >400 W 
Peak Power2  >120 kW  >310 kW  >120 kW  >50 kW 
Tuning Range4  700–1020 nm 

　
Mai Tai Broadband Specifications
Output Characteristics1 
Mai Tai BB Performance  710 nm  800 nm  920 nm  990 nm 
Average Power2  >700 mW  >1.5 W  >650 mW  >250 mW 
Peak Power2  >85 kW  >185 kW  >80 kW  >30 kW 
Tuning Range4  710–990 nm 

　
Mai Tai XF Specifications
Output Characteristics  
Wideband Mai Tai Performance  710 nm  800 nm  950 nm 
Average Power2  >700 mW  >1.5 W  >500 mW 
Peak Power2  >85 kW  >185 kW  >60 kW 
Tuning Range4  710–990 nm 

　
Mai Tai XF-1 Specifications
Output Characteristics  
Single Diode Wideband Mai Tai Performance 1  710 nm  800 nm  920 nm 
Average Power2  >400 mW  >900 mW  >400 mW 
Peak Power2  >50 kW  >110 kW  >50 kW 
Tuning Range4  710–920 nm 

　

選配 B :  Coherent Chameleon 系列 超快雷射系統 ( Ultrafast Ti:Sapphire Laser System )
Coherent Chameleon Chameleon-XR
Output Power (W) 1 1.5
Tuning Range (nm) 720-950 705-980
Pulse Width (fs) <140 <140

Beam Routing & EOM system ( for IR, Multi-Photon system )
Beam Routing Module & EOM system, ( for DM Upright Microscope )
Beam Routing Module & EOM system, ( for DMI Inverted Microscope )
Additional Detection PMT ( NDD, TLD, RLD ) for Multi-Photon Imaging System　
TLD 1 channel for DMI
TLD 1 channel for DM
TLD 2 channels for DMI
RLD 1 channel for DMI
RLD 1 channel for DM
RLD 2 channels for DMI
RLD 2 channels for DM
Filtercube NDD 2 ch. DAPI-FITC
Filtercube NDD 2 ch. DAPI-TRITC
NDD detection unit
NDD 2x4 Main Module
Channels 1-2 (RLD or TLD) for 2x4
Channels 3-4 (RLD or TLD) for 2x4
Liquid Light Guide RLD for DMI
Liquid Light Guide TLD for DMI
Liquid Light Guide RLD for DM
Liquid Light Guide TLD for DM
External Port ( optional ) and Filter cube combination
X1 Port Emission Access
Filterwheel and Analysator rot.
X1 port adaptation for APD detection
X1 port adaptation multipurpose
X1 port adaptation for FLIM
X1 port adaptation for ROI Spectrometer
Filtercube FCS Neutral
Filtercube FCS GFP/RFP wide
Filtercube FCS Alexa 488/633
Filtercube FCS CFP/YFP
Filtercube FCS CFP/RFP
Filtercube FSC GFP/RFP narrow