Journals are similar to a macro and they record the actions of a MetaMorph user. They can be executed by other journals and can be edited.

The software includes standard interfaces for illumination, stage, and Z-motor devices, and it provides digital I/O to communicate with unique devices.

• Control the illumination wavelength, employing filter wheels, shutters, liquid crystal tunable filters, monochromators, or other wavelength selectors
• Synchronize image acquisition and analysis with the opening and closing of shutters
• Scan slides or multi-well plates with a motorized stage
• Acquire images of a through-focus Z-series for subsequent deconvolution and 3D reconstruction
• Control custom devices that accept commands through a serial or parallel port connection
• Auto-focus at multiple stage positions or at specific intervals in a time lapse environment

• Rotating objectives, change filter cube settings, open and close the shutter, switch the illumination lamp on or off, and change the lower and upper prism positions
• Controlling the X, Y, and Z-axis positioning of a stage, move the stage, and store the positions for future use
• Controlling automated stages to capture multiple locations in a sample or multiple samples, including dishes and multi-well plates
• Using focus motors to collect Z-series or through-focal image sets
• Acquiring images using eight different groups of settings for wavelength, intensity, and exposure duration
• Acquiring a spectral scan in a range of excitation or emission wavelengths
• Capturing a time lapse series of images at specified intervals and durations

Standard Features  ( with MetaMorph Premier and Basic systems)　

• Binary and 8-, 16-, 24-, 48-bit image display and processing
• Stack display and processing including best focus, montage, topographical surface, view orthogonal planes, and create movie
• Over 20 morphology filters, including erode, dilate, skeletonize, outline, and segment
• Arithmetic operations including add, subtract, multiply, divide, logical AND, OR, XOR, minimum, maximum, and average
• Fast Fourier Transform (FFT)
• Shading correction and background subtraction
• Filters including sharpen, low pass, detect edges, unsharp mask, median filter, remove haze, and custom kernels
• 2D deconvolution ( nearest neighbors and no neighbors)
• 3D reconstruction
• FRET
• Auto expose from digital cameras
• Time lapse acquisition
• Spectral scan acquisition
• Z-series acquisition ( with Z-motor driver )
• Morphometry and distance measurements including area, hole area, standard area count, perimeter, centroid X and Y, Z-position, width, length and height, orientation, breadth, fiber length, fiber breadth, shape factor, elongated form factor, inner radius, outer radius, mean radius, equivalent radius, equivalent spheroid volume, equivalent prolate volume, equivalent oblate volume, equivalent sphere surface area, average, gray value, total gray value, optical integrated density (OD), integrated OD, intensity center X and Y, radial dispersion, texture difference moment, texture inverse difference moment, OD variance, OD relative low/medium or high areas, OD relative low/medium or high amounts, OD relative low/medium or high distances, and others including over 30 elliptical Fourier parameters to assist in defining shape analysis patterns
• Color-combine and separate 24-bit or 48-bit images
• Regions of interest tools
• Image histogram
• Data logging and exporting to text file or spreadsheet programs that support DDE (Dynamic Data Exchange) such as Microsoft® Excel
• Automation through journals and taskbars
• Customizable toolbars and windows
• Presentation features including organize and label with text, date, time, or calibration bars, convert color images to monochrome, produce intensity profile images, create image montages and movies, overlay images, and document the operations performed on the images

Microscope Control Options ( 顯微鏡自動化控制功能 )

• Most popular automated microscope models from Leica, Nikon, Olympus, and Zeiss
• Digital auto-focus
• XY stage device control for popular models from major manufacturers
• Z-axis device control for popular models from major manufacturers
• Piezo-actuated Z and XY device control
• Monochromator control for illumination
• Filter wheel and shutter control
• UniBlitz® shutters
• AOTF for laser control
• Liquid Crystal tunable filters
• Custom I/O (RS-232 serial and TTL parallel)

Acquisition Options ( 影像擷取控制選配 )

• Digital CCD cameras, both monochrome and color, including: cooled, full frame, frame transfer, interline, back thinned, intensified, and on-chip multiplication gain, from major manufacturers
• Video cameras, both monochrome and color, including: RS-170, CCIR, on-chip integration, intensified, CCD, and tube, from major manufacturers
• Simultaneous acquisition from two cameras or control of an image splitting device for projection of two or four emission wavelengths onto a single camera (appropriate hardware required)
• Wavelength streaming and/or Z-axis streaming ( 專利設計 )

Optional Features for Basic Systems  ( MetaMorph Premier systems 包括此部分軟體功能 )

• Multi-dimensional imaging
• Overlay multi-fluorescent images
• Image stitching
• Motion analysis and particle tracking
• Colocalization and correlation measurements

Additional Modules ( 選配軟體模組 )

• 3D deconvolution
• Advanced 3D visualization
• Neurite outgrowth