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Roper Scientific

Cascade Cooled CCD Camera

超高解析

超高靈敏

超高速率

Roper 最新的 Cascade II 512 採用最新的 EMCCD - ON-Chip-Multiplication Gain 技術, 無須外接的冷卻裝置, 可降溫至攝氏 - 80 度, 將 CCD 暗電流 ( Dark current ) 降到最低, 獨家的真空封裝技術, 能提高 QE 值, 確保 CCD 使用效率與壽命, 16 bits ( 512 x 512 pixels ), Frame-Transfer ( back - illuminated ), 最大量子峰值效率 ( QE ) 高達 92% 以上. 三段讀出速率 10, 5, 1 MHz 提供不同的應用需求.

廣泛的建議應用範疇, 包括 " 極弱低光影像擷取應用 " :

......... Ultra-low-light fluorescence

......... Total internal reflection fluorescence microscopy

......... Single-molecule fluorescence

......... Spectral imaging

......... Bio-Luminescence, Chemo-Luminescence

......... FRET, GFP Fluorescence imaging

美嘉儀器是台灣地區獨家代理行銷與提供服務 Cascade 系列 Cooled CCD Camera.

Cascade 採用全世界獨家的 CCD 技術, 提供極高的感測靈敏度, 極小的訊號可被放大, 所以, 極弱, 極快的訊號, 都可被偵測. 其 QE > 90% 以上 ..........

請參考 On-Chip Multiplication Gain 技術 ( PDF 下載 )

Cascade 是你的高階 CCD Camera 的唯一正確選擇 !
Cascade 系列 CCD Camera 堪稱全世界最高階的 CCD 攝影器, 基本上, 極弱的訊號, 極低的螢光, 極高速的動態反應, 都可適用. 我們在此推薦適當的主要應用如下表所列 : ( 實際選配必須依您的實驗應用需求而定, 請洽本公司專業技術人員 )
*Cascade*	1K	128+	128	512B	512F	650
spinning-disc confocal microscopy	V	V	V
high-resolution GFP / FRET detection	V	V	V	V	V	V
Live-cell time-lapse applications	V	V	V	V	V	V
polarization / anisotropic imaging	V
Neurosciences		V	V
Single-Molecule Fluorescence		V	V	V	V	V
Ca⁺⁺ratio imaging			V	V	V	V
Fixed cell fluorescence	V	V	V	V	V	V
Intracellular ion imaging			V	V	V	V
Biological fluid flow measurements						V
Microspectroscopy				V

機型	Cascade 1K	Cascade 128+	Cascade 128	Cascade 512B	Cascade 512F	Cascade 650
On-Chip Multiplication Gain	V	V	V	V	V	V
On-Chip Multiplication Gain	min. gain 200x	1000X ( 4096 step )	1000X ( 4096 step )	1000X ( 4096 step )	1000X ( 4096 step )	min. gain 200x
Frame Transfer	V	V	V	V	V	V
Sensor	TC285	e2v CCD60	e2v CCD60	e2v CCD97	e2v CCD87	TC253
CCD Chip	Front-illuminated	Back-illuminated	Back-illuminated	Back-illuminated	Front-illuminated	Front-illuminated
CCD format	1004 x 1002	128 x 128	128 x 128	512 x 512	512 x 512	653 x 492
Linear full well	30,000 e^-	750 K e^-	750 K e^-	800 K e^-	800 K e^-	27,000 e^-
Pixel size	8 x 8 µm	24 x 24 µm	24 x 24 µm	16 x 16 µm	16 x 16 µm	7.4 x 7.4 µm
Imaging area	8 x 8 mm	3.072 x 3.072 mm	3.072 x 3.072 mm	8.2 x 8.2 mm	8.2 x 8.2 mm	4.9 x 3.7 mm
Digitizer	16 bits @ 10 Hz	16 bits @ 12 Hz	16 bits @ 10 Hz	16 bits @ 10 Hz, 5 MHz, 1 MHz.	16 bits @ 10 Hz, 5 MHz, 1 MHz.	16 bits @ 10 Hz, 5 MHz,
Readout noise ( on-chip )	< 1 e^-rms	< 1 e^-rms	< 1 e^-rms	< 1 e^-rms	< 1 e^-rms	< 1 e^-rms
Readout noise	< 15 e^-rms @ 10 MHz	< 65 e^-rms @ 12 MHz	< 60 e^-rms @ 10 MHz	< 60 e^-rms @ 10 MHz	< 60 e^-rms @ 10 MHz	25 e^-rms @ 10 , 5 MHz
Frame readout	110 ms/frame, 1 ms	83 nsec / row	100 nsec / row	2 µsec / row	2 µsec / row	36.2 ms/frame, 210 ms
Binning	1 - 4 binning	1 - 4 binning	1 - 4 binning	1 - 4 binning	1 - 4 binning	1 - 4 binning
Max. frame rate	103 fps	4149 fps	3226 fps	329 fps	329 fps	555 fps
CCD Temperature	- 30^oC	- 30^oC	- 5^oC	- 30^oC	- 30^oC	- 25 - 35^oC
Dark current	0.5 e^-/p/s @ -30^oC	< 1 e^-/p/s @ -30^oC	11 e^-/p/s @ -5^oC	1.0 e^-/p/s @ -30^oC	1.0 e^-/p/s @ -30^oC	1 e^-/p/s @ -35^oC
C-Mount	V	V	V	V	V	V
PCI interface	V	V	V	V	V	V
PVCAM	V	V	V	V	V	V
Software	V	V	V	V	V	V

5 個選用 Cascade 的理由 :
1	Highest Sensitivity ! ( 超高靈敏, 超高解像力, 超高 QE 值 ) Photometrics® Cascade cameras offer unmatched sensitivity thanks to on-chip multiplication gain, an innovative CCD technology that multiplies low-light-level signals above the read noise of the system. To compound this benefit, the Cascade:512B and 128+ models utilize back-illuminated detectors that deliver the highest QE available. The newest Cascade: 1K utilizes advanced virtual phase CCD technology and achieves better UV to NIR sensitivity than any front illuminated CCD technology.
2	"Two-in-One" Versatility ! ( 獨家技術 ) Cascade:512B and Cascade:512F models are offered with dual amplifiers to ensure optimal performance for ultra-low-light imaging and wide-dynamic-range applications. Either one of these cameras provides a high-performance solution for single-molecule fluorescence (SMF) as well as brightfield / fluorescence imaging applications.
2
3	Rugged Durability ! ( 攝影器穩固, 取代 I-CCD ) Traditional ICCD cameras use external image-intensifier hardware to amplify low-light-level signals. Unfortunately, these intensifiers are susceptible to damage in bright-light conditions and suffer from lower resolution and uniformity. By contrast, Cascade cameras do not use external image-intensifier hardware. Charge is multiplied right on the CCD — in the solid-state domain! Cascade CCDs offer superior resolution and uniformity and are not prone to damage from bright light.
4	Supravideo Speed ! ( 超高速影像擷取 ) Until now, selecting a CCD camera for low-light imaging required giving up speed. Although capable of detecting low-light signals, slow-scan cameras could only produce 1 to 4 frames per second. However, by effectively minimizing read noise, on-chip multiplication gain allows Cascade cameras to deliver full-frame images at video rates! (The use of ROI boosts these rates into the range of hundreds of frames per second!) In addition, the frame-transfer architecture of Cascade CCDs provides the 100% duty cycle required for continuous observation of kinetic events (e.g., motion or tracking). The new Cascade: 128+ delivers unprecedented 510 frames per second at full resolution for ultra high-speed imaging.
5	Global Compatibility ! ( 被許多軟體支援, 供應支援介面 PVCAM ) All Cascade cameras utilize Roper Scientific’s renowned PVCAM® application programming interface. This intelligently designed API enables your camera to be operated with a multitude of popular image-processing software programs. PVCAM offers the broadest flexibility on the market!
相關應用連結 ( from Roper Scientific ) - 值得參考 !
	Mesoscopic and Nanoscopic Materials Characterization using 1:1 fiber coupled, PI-SCX: 4300 camera Selecting a System for Raman Spectroscopy (pdf) ( 拉曼光譜適用的 CCD Camera ) BALF Research with Raman Spectroscopy (pdf) ( 拉曼光譜應用 ) Geoscience Applications of X-Ray Computed Microtomography at a Third-Generation Synchrotron (pdf) High-Energy X-Ray Nondestructive Testing (pdf) Intensified CCD Imaging and Spectroscopy Unravel the Mysteries of Carbon Nanotube Formation (pdf) ( 積分式 I-CCD camera 的光譜應用 ) Measurement of Environmental Contaminant Hydrocarbons by Fluorescence Spectroscopy (pdf) Planar Laser-Induced Fluorescence Imaging of OH Radicals (OH-PLIF) in Hypervelocity Combustion with an Intensified CCD System (pdf) Real-Time Xylene Isomer Quantification Using Chemometric Raman Analysis (pdf) Time-Resolved Fluorescence Spectroscopy (pdf) ( 建議研讀 ) Using Planar Laser-Induced Fluorescence To Study Plasma Turbulence (pdf) ( 建議研讀 ) X-Ray Photon Correlation Spectroscopy at a Third-Generation Synchrotron (4MB pdf)