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| 美國
Intelligent
Imaging
Innovations
公司的高階顯微鏡專用數位影像軟體,
提供了創新與實用的完整性能.
其產品之一
SlideBook
軟體, 能驅動幾乎所有的 CCD
攝影機,
控制各廠牌的顯微鏡及所有顯微鏡自動化的周邊設備,
包括電控載物台,
濾鏡
轉盤, 電控調焦,
光波切換光源,
單光儀....等.
軟體應用提供了多維影像擷取與分析
( 6D,
Multi-Dimensional
Imaging ), 長時間活細胞螢光影像擷取
( Time lapse ),
螢光及干涉影對重疊
取向, 及 螢光離子流影像, FRET, FRAP 及
Densitometry,
Tracking, Multi-Well Cell Imaging Screen
支援雷射切割,
共軛焦系統..
等高階應用.
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按我們長期在影像系統的經驗,
SlideBook 有下列優點 :
1. 軟體應用功能最完善
(
從研究人員的觀點作軟體設計
)
2. 軟體開放式架構最具人性化
( 可使用於
Windows 2000 / XP 及 Mac
系統 )
3. 使用非常簡易
(
使用人員不用浪費時間在學習操作上,
能輕易入門與進階使用 )
4. 價位組合最具合理
(
最合理的價格與性能效益比 )
5. 無限擴充性
( 可合符現在與未來的需求
) |
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SlideBook V. 4.0
的主要功能
|
Standard
Features ( 標準性能 )
|
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-
Intuitive Slide Interface
-
Open
-
Close
-
Save
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-
Automated Microscopes
-
Fluorescence Shutters
-
Bright Field Shutters
-
Filter Wheels, Turrets, and Monochrometers
-
Objective Turrets
- X,
Y Stages
- Z
Axis Control Systems
- Mag
Changer / Optovar
-
Prism Turrets
-
Port Controls
-
Lamp Intensity
- LCD
Filters
-
Imaging Splitter
|
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-
Controls Most Scientific-Grade Digital
Cameras
-
Simultaneous Dual-Camera Capture
-
Interactive Control of Speed, Gain, and
Intensification (when possible)
|
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- 2D
(Single and Multi-Channel)
- 2D
Time Lapse
- 3D
(Single and Multi-Channel)
- 4D
(3D Time Lapse)
-
Automatic Objective, Filter, X-Y-Z
Location Detection and Capture Time
-
Image Annotation and Naming
-
Interactive Annotation Buttons
-
Autofocus
-
Live Region Tracking
-
Multi-Point (x, y, z)
-
Mid-Volume Capture
-
Mixed Brightfield / Fluorescence Capture
-
User Controlled Capture Feedback
- Single
Channel
- Color
Composite View
- Histogram
|
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-
Image Renormalization
-
Linear Filtering
- 2D
Deconvolution
- No
Neighbors Deconvolution
-
Channel Math
-
Image Math
-
Flat Field Correction
-
White Balance
- FFT
-
Photobleach Correction
|
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-
Interactive Montage Boundary Selection
-
Automatic Stage Movement
-
Automatic Image Alignment
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- ROI
Selections
-
Laser Power Control
-
Laser Pulse Rate Control
-
Hardware Control During Image Capture
- Cell
Selector Module
- Automated
Fragment Retrieval (AFR) Module
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- RGB,
GrayScale, Inverse Grayscale, Pseudocolor,
Gated Pseudocolor
-
Tile View (3-D, Time Lapse, 4-D)
-
Three View (3-D, Time Lapse, 4-D)
-
Main View (Axis Selection)
-
Channel View
-
QTVR Volume Rendering
-
QuickTime Movie Generation (3-D and Time
Lapse)
-
Isosurface Rendering
-
Surface Plot
- 3-D
Prism Generation
- 3-D
and 4-D Data Rotation
-
Path Sectioning (Time Lapse)
- 4D
View
- 4D
Tile View
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-
Intensity Segmentation
-
Gradient Segmentation
-
Magic Tools for Local Segmentation
-
Manual Mask Editing
-
Binary Operations (AND, OR, NOT)
-
Erode
-
Dilate
-
Skeletonize
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-
Volume
-
Surface Area
- 3D
Center of Volume
- 3D
Center of Intensity
- 3D
Integrated Intensity
- 3D
Mean Intensity
- 3D
Line Intensity Plot
- 3D
Distance (Linear and Non-Linear)
- 3D
Object to Object Distance Calculation
|
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-
Plug-in architecture for end-user writable
features
-
Automatic methods for generating and
storing statistics
-
Advanced clustering techniques
-
Many new statistics, including:
-
cross-channel colocalization
-
skeleton-based features
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-
Area
-
Integrated Intensity
-
Average Intensity
-
Intensity Deviation
-
Center of Mass
-
Velocity
- 2D
Object Counting
- 2D
Object Distance Measurement
-
Colocalization
-
Distance (edge to edge)
-
Distance (center to center)
-
Intensity Plot
-
Angles
|
- 3D
Higher Order Feature Segmentation
|
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-
Single Channel
-
Color Composite View
-
Histogram
|
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-
TIFF, PICT, raw, QuickTime, FluoView,
Zeiss 510LSM, BioRad PIC, and more
|
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-
User Definable Park Channel for Spinning
Disk
-
Laser Power Control
-
Laser Sleep Mode Control
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Additional Modules (
選配軟體模組
)
|
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-
Nearest Neighbor
- Multiple
Processor Optimization
- Speed
Optimized
-
Constrained Iterative
- Noise
Smoothing
- Edge
padding
-
Individual Channel Programmability
- PSF
Guide
- PSF
Database
-
Uses Calculated or Measured PSF's
-
Speed Optimized
-
Programmable Batch Deconvolution
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- TTL
Control Hardware
- TTL
Start of Image Capture
-
Pushbutton Control of TTL Out During Image
Capture
-
Automatic TTL Pulse During Image Capture
-
Record External TTL Pulses during Image
Capture
-
Includes Hardware for Pulse Generation and
Recording
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Correlate Pathways in 4D
-
Manual or Automatic Path Delineation
-
Combine Manually and Automatically
Delineated Paths
-
Tracks Small and Large Objects
-
Track Objects by User-Defined Feature
(i.e. displacement, volume, etc)
-
Identify or Edit Merges and Divisions
-
Object to Object Correlations
-
Open-GL Based 3D Path View for Visualizing
Trajectories Over Time
- 4D
Statistics
- Average
Velocity
-
Instantaneous Velocity
-
Acceleration
- Angular
Momentum
- Level of
Randomness in an Object's Motion
- Custom
Statistics Through Feature Engine
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-
User Selectable Configuration
-
Well Visitation
-
Visitation Frequency
-
Integrated Autofocus Per Well
-
Image Storage by Well
-
Ability to Save Expirement Template Files
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-
Ratio Capture and Analysis
- Real-time
Ratio Region Tracking and Graphing
- Regions to
Mask Transfer
- Supports
Simultaneous Ratio Capture (e.g. FURA-2
and BCECF)
- FURA-2
Calibration Guide
- Immediate
Display of Automated Ratio Values or Ion
Concentration
- Analytical
Graphing Tools
- Multiple
Camera / MultiSpec Imager enabled
-
Acceptor Photobleach FRET Capture and
Analysis
- Automatic
Bleach Capture Script
- Automatic
FRET Computation
- Gated
Pseudocolor Display
- Track FRET
by Object or Pixel
- Background
Subtraction
-
Sensitized Emission FRET Capture and
Analysis
- Live
Corrected FRET Image Generation
- Background
Subtraction
- Channel
Bleedthrough Calculation
- FRET
Calibration Guide
- Gated
Pseudocolor Display
- Track FRET
by Object or Pixel
-
Densitometry Capture and Analysis
-
Interactive Calibration Guide
- Multiple
Curve Fits
-
Calibrations can be Saved and Applied to
Multiple Images
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Train Custom Neural Network Kernels
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Ability to Save Kernels and Apply to New
Images
-
Whole Cell and Subcellular Object
Recognition by Morphometry
-
Whole Cell and Subcellular Object
Recognition by Channel Correlation
-
Supports 2D, Time-lapse, 3D and 4D Data
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3D Deconvolution module |
|
The 3D
Deconvolution module adds a 3D deblurring and an
image restoration algorithm to SlideBook. The
nearest neighbor and constrained iterative
deconvolution algorithms have been optimized for
speed and performance. The module includes an
interactive guide for measuring point spread
functions (PSF) and a PSF database. The database
can store multiple PSFs for each configuration,
allowing easy selection of the appropriate PSF. |
Nearest Neighbors Deconvolution
Our Nearest
Neighbors deconvolution is a rapid way to
“deblur” fluorescence data. The algorithm uses
the plane above and below the plane of interest
to compute and subtract the fraction of the data
that is out-of-focus information. |
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Constrained Iterative Deconvolution
Our
Constrained Iterative (CI) deconvolution is a
true image restoration tool. Based on the
algorithm developed by David Agard at UCSF, our
CI deconvolution can quantitatively reassign
out-of-focus information in 3-D data while
improving both axial and lateral data
resolution. |
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The module can use
either measured point-spread
functions (PSFs) or
computed PSFs when measured PSFs are
unavailable. The module includes a PSF collection guide
and a PSF database. Given SlideBook’s knowledge of image
collection optics, the correct PSF can automatically be
applied to CI deconvolution without user assistance.
With SlideBook 4.0,
CI deconvolution includes extensive speed improvements
taking full advantage of advances in computational
algorithms, multiple processors and hardware
acceleration for both Macintosh and Windows platforms.
CI deconvolution has been improved so that it can handle
deconvolution of very large data sets (up to 350 MB per
wavelength for a system with 2 GB RAM). In addition, CI
deconvolution now includes a number of advanced user
options to compensate for a variety of imaging
conditions, from single cells to tissue sections, so
that an optimal deconvolution can be achieved for a
particular experiment.
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FRET /
Ratio / Densitometry (FRD) module
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This module
is specifically designed to handle data that is
only meaningful in the context of other data.
Examples of such data are FRET where the data
are represented as a percentage of a donor
molecule or FURA-2 where the data is meaningful
as the ratio of emissions of two separate
excitations. |
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The FRD
module for SlideBook™ 4.0 is the first
commercially available software specifically
designed for FRET analysis. This revolutionary
software was developed in collaboration with a
number of leading biologists whose laboratories
focus on FRET. The FRET module contains analysis
tools for both sensitized emission FRET and
acceptor photobleaching FRET. These tools
include: |
-
Channel Bleedthrough Calculation
-
Background Subtraction
-
Corrected FRET Image Generation (Herman
Equation)
-
Single Pixel to Whole Object Energy Transfer
Measurement
-
Support for 2-D, 3-D and 4-D Data
|
The module
can be combined with image capture and other
on-line modules for real-time FRET analysis. In
addition, SlideBook™ supports dual camera
capture and the Multi-Spec Microimager device
from Optical Insights for true real-time FRET
image acquisition |
The
measurement of free ion concentrations in living
cells provides insight into cellular processes such
as activation and compartmentalization. Ratiometric
ion indicators change their excitation or emission
profiles when bound to specific ions. These
indicators allow the real-time measurement of ion
concentrations in living cells. :This module
supports a wide array of ion indicators including
FURA-2, Indo-1 and BCECF. The module includes a
variety of specific features including:
-
Real-time ratio computation
-
Real-time graphing of ratios and/or raw channels
-
Real-time tracking of up to 20 ROI
-
Simultaneous imaging of two ratio indicators
-
Calibration guide for computing nM calcium
concentration by pixel
-
Analytical Graphing tools
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Particle Tracking Module
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The
evolution of digital microscopy has placed
increasing emphasis on the quantitative
information contained within collected images.
The Statistics Engine for SlideBook™ has been
completely reworked for version 4.0 to provide
researchers with a complete suite of analytical
tools specifically designed for quantifying data
from microscopes.
The new
Statistics Engine includes:
- Plug-in
architecture for end-user writable features
-
Automatic methods for generating and storing
statistics to aid in gathering large amounts
of data
-
Advanced clustering techniques to help define
populations of objects and images
- Many
new statistics, including cross-channel
colocalization and skeleton-based features
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| The all
new 4D analysis and particle tracking module for
SlideBook 4.0 contains powerful tools tracking
individual 3D objects in 4-D datasets. The
particle tracking engine is based on individual
object paths. Individual objects will have a
unique path through time and can therefore be
followed.The particle tracking module can also
correlate objects with respect to one another
and generate statistics about each object at
each time-point.
Object paths
can be traced either automatically using
computational algorithms or through manual
delineation. The particle tracking module also
offers complete flexibility for combining both
automated and manual tools to optimize path
detection for any given sample. |
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Once
object paths have been traced, there are a
variety of visualization tools to help explore
the objects. In the OpenGL-based 3DpathView, for
example, an individual path can be selected and
individually rendered over time. This rendering
can be rotated freely and played as a movie (in
either direction) or in single frame increments.
As users explore the data, they can manipulate
the path, either to prune a split/merge as
objects move, or get statistics for a given
object.
The
statistics generated by the particle tracker
include everything from velocity and
acceleration of objects to a description of
randomness of an object's motion. These
statistics can be automatically generated for
all objects/paths or statistics can be generated
on a path-by-path basis. In addition, all
delineations, processes and analyses in the
particle tracker are automatable. Each step in
the tracking scheme can be stored and retrieved
from a methods database, so that a series of
commands can be easily written and retrieved for
use in future experiments. |
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Multi-Well Plate Module
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SlideBook™ 4.0
features a new, easy-to-use lpate reading module. This
module enables SlideBook™ to capture data from a wide
range of multi-well plates, including 24, 96, and
384-well plates.
This module gives
the researcher complete control over all imaging
parameters. An expirement can include any combination of
SlideBook™-supported imaging modes, including
multi-spectral fluorescence and combination
fluorescence/transmitted light imaging. In addition,
this module includes an optimized auofocus algorithm to
ensure high quality image data. The researcher can
select the range of wells to be images, along with the
location and number of images to be taken per well.
These parameters can be saved as a template, making it
fast and easy to image multiple plates under identical
conditions. |
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SlideBook 4.0 是最具企圖心的軟體,
其功能幾乎涵蓋 UIC
的 MetaMorph 及
MetaFluor 以上. 同時,
也包括許多
Leica FW4000
軟體的優點. 除了上面所列的功能外,
尚有許多選配功能供選用.
從今年 5 月引進台灣起,
已在國內一些單位獲得數套訂單 (
如台大醫學院, 陽明大學
... 等 ).
您只要提出您的應用需求,
我們就會盡全力去提供令您滿意的結果.
建議您搭配使用
Leica 新一代的微電腦控制數位顯微鏡
DM
4000B, DM 5000 B, 或者,
Leica DM LA,
DM RA2 (
正立 ),
倒立顯微鏡建議使用
DM IRE 2. |
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Leica DM 5000B |
Leica DM IRE 2 |
Leica DM RA2 |
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其他相關資料下載及連結 |
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