美嘉儀器股份有限公司

徠卡顯微鏡系統

雷射掃描共軛焦顯微鏡

半導體及工業用顯微鏡

顯微數位影像系統

頻譜螢光雜染影像比對系統 - FISHView EXPO

ASI FISHView EXPO 是最專業的 FISH 定量分析, 比對, 臨床資料庫管理的影像系統. 完全支援 CytoLabView 染色體比對系統 ( SKY ). ASI 同時提供染色探針 ( Chromosome Paiting Probes )

FISHView ( Fluorescence In-situ Hybridization ) 頻譜螢光雜染影像比對系統

FISHView EXPO

Spectral FISH

FISHView EXPO 可適用於所有廠牌顯微鏡,.


明視野 FISH 影像	螢光影像	訊號精確分析	比對分析

染色體探針 ( Chromosome Painting Probes )


單一特定螢光標定探針 ( Probes - Green / Red )

三種螢光雜染探針 ( Probes )


	Centromeric probes also called: CEP probes (Chromosome Enumeration Probes)
The first probes used for FISH analysis comprised of DNA sequences for the centromeric area of each chromosome. This region consists of a sequence of DNA that is repeated hundreds of times. This class of DNA probes is suitable for the analysis of numerical aberrations such as trisomy (the presence of an extra chromosome) and monosomy (the absence of a single chromosome). One of the important advantages of FISH over conventional cytogenetic techniques is the ability to visualize the signals through all stages of the cell cycle and not only in dividing cells at the metaphase stage. This approach is termed "interphase cytogenetics". The advantages of "interphase FISH" for the diagnosis of numerical aberrations are obvious. The technique is fast and the signals can be visualized on different kinds of cells : fetal cells, embryonic cells, cancer cells and more. The signals are easily detected compared to the assessment of chromosomal bands. However, since the whole karyotype is not visible, we can detect only specific aberrations according to the probes that were used in the analysis. In tumor analysis interphase aberrations detection is of great importance due to the difficulty in producing metaphase spreads that are representative of the whole population. Therefore, FISH has already become an important tool in tumor analysis for the detection of numerical aberrations that are of prognostic important.
	unique DNA sequences also called LSI (Locus Specific Identifier Probes)
The second group of probes are the unique sequences probes. These are single copy sequences that can range from very small probes (approx. 1 Kb- 1000 base pairs of DNA) to a very large ones which consist of hundreds of Kb. These region specific probes can be used for diagnosis of specific translocation, cryptic translocation in the telomeres and detection of deletions in prenatal diagnosis and in cancer analysis. These probes can be used either in interpahse or in metaphase.
	painting probes also called WCP (Whole Chromosome Paints Probe)

The third group of probes are the painting probes. These are composite
probe sets specific for the entire chromosomes. This type of probes enable
the whole chromosome to be stained. The painting probes are mainly useful
for the detection of structural chromosomal aberrations like translocation
only in metaphases. Painting probes have also proven useful for the
identification of marker chromosomes (that is a chromosome that is
different from any normal chromosome in the karyotype).

The use of painting probes is also very useful for the detection of
reciprocal translocations after exposure to ionizing radiation., in
mutagenesis studies and chemical dosimetry.

ASI can supply right away all the 24 individual human chromosomes or
the 21 mouse chromosomes labeled in Green or in Red. We can supply each one
in a separate kit for 10 slides or we can supply them in combination of two
chromosomes together: one in Red and one in Green. We can also supply a kit
with three chromosomes: one in Red , one in Green and one in Yellow.

專業 FISH 系統, 沒有其他廠商可比擬

染色體比對功能

影像處理, 個別放大, 分離, 強化... 等.


單一視窗螢幕下, 做完全的操作, 使用簡易.

專業的報告製作

FISHView EXPO 性能說明

Powerful Multi-layer Imaging

Original 12-bit images always preserved

Time resolved imaging for a sequence of filters to see changes in a sample over time

Automatically converts sequences of images at different focal planes into one focused image

Support for unlimited number of filters and automated filter wheel

Manual and automatic filter wheel and microscope control

Automatic exposure control and image enhancement

Robust Image Analysis

FISH signal quantification for size, length, and intensity of each layer

Extensive color control capabilities

Real-time updated gallery of acquired layers

Corrections for shift between layers

Manual and automatic segmentation

Automatic background correction

User-friendly Reports and Comparison Tables

Multi-image reports (up to 24 images in one page)

Ready-to-use report templates

Flexible image annotation

Robust comparison tool to compare images within a case and between cases

Complete Cytogenetics Tool Kit

Full karyotyping support

Automatic contrast for counterstained and probe signals

Image gallery to view cases by metaphase or karyotype

Dynamic karyotype tables which automatically change to accommodate changes in species, chromosome size, and your personal settings

Seamless upgrade to BandView and SKY

Multi-case chromosome comparison, combining G-band, FISH and SKY results

	使用 SKY 發表的文獻索引 ( 部分索引 )

Applied Spectral Imaging ( ASI - 以色列 ) 產品一覽表

CytoLabView

微電腦控制, 數位頻譜影像技術

請點選下列 ASI 產品資料說明 .....


BandView EXPO G, R, Q, DAPI-Banding 染色體比對	FishView EXPO 染色體, 細胞基因之 FISH 影像擷取分析	SKYView EXPO 染色體 SKY ( Spectral karyotyping ) 彩色比對, 計數及影像及資料管理中心


CGHView EXPO 染色體 CGH 分析	SpectralView EXPO 頻譜影像系統	Case Data Manager EXPO 影像及病例資料管理中心


SkyPaint probes 染色體雜染探針試劑	Single paint probes 單一特定染色體探針試劑	SpectraCube 頻譜影像擷取處理器

美嘉儀器股份有限公司 Major Instruments Co., Ltd.

台北總公司 : TEL : 02-2808-1452 FAX : 02-2808-2354 維修中心 : 02-2808-2353 高雄辦事處 - TEL : (07) 725-8177 FAX : (07) 725-8204

E-mail : major@major.com.tw http://www.major.com.tw