徠卡顯微鏡系統
雷射掃描共軛焦顯微鏡
半導體及工業用顯微鏡
顯微數位影像系統
細胞基因影像分析系統
其它光電微機電系統
其它生醫研究儀器
維修與技術支援服務
聯絡與留言
神經與細胞膜電位紀錄與光譜影像系統
美嘉儀器公司從事動物生理, 藥理, 毒理, 心理, 行為研究設備, 以及野生動物的無線電與衛星追蹤系統, 迄今, 已超過 17 年. 我們仍然秉著專業與熱誠的服務態度, 持續引進專業性與實用性的研究儀器與技術.

目前, 我們更專注於電生理紀錄與光譜影像的系統整合與技術推廣. 我們與學界的合作, 已經成功的建立 :

螢光離子流影像與胞內紀錄 ( Fluorescence Ratio Imaging & Single-Channel Recording )
細胞與腦切片的紅外光影像與膜電位紀錄 ( Infrared Patch-Clamp Recording & Imaging )
心臟心電 ( 神經電位 ) 紀錄與動態光譜顯像分析 ( Cardiac & Neuron Optical Mapping )
活細胞紀錄應用之共軛焦與多光子影像系統 ( Confocal & Multi-Photon Imaging System )
顯微鏡 ( Optical Microscope & Fluorescence Stereomicroscope )
Leica DM LFSA

載物台固定, 鼻輪升降式顯微鏡 ( Fixed stage microscope )

全世界獨一的獨立外接顯微鏡控制器, 沒有電場與震動干擾.

適合電生理與共軛焦系統.

Leica DM IRE 2

微電腦控制倒立顯微鏡

全世界獨一的獨立外接顯微鏡控制器, 沒有電場與震動干擾.

適合電生理與共軛焦系統.

Leica Fluo Combi

螢光實體顯微鏡

全世界獨一的獨立外接螢光實體顯微鏡, 分離式控制器, 同時具有實體物鏡與光學高倍鏡頭 ( 10x / 20x )沒有電場與震動干擾.

適合實體觀察與紀錄, 電生理與共軛焦系統.

雷射掃描共軛焦與多光子影像系統 ( Confocal  & Multi-Photon Imaging System )
Leica TCS SP2 RS with TCS MP ( Multiphoton ) 高速掃描之光譜式共軛焦顯微鏡, 內建多光子影像系統. 建立活細胞影像與電生理 ( Patch-clamp )紀錄系統.
膜電位紀錄放大器與訊號分析 ( Patch-Clamp Amplifier & Data Acquisition )

 

 

美國 Warner 儀器公司是近十年來成長快速的公司之一. 早期主要產品已 Oocyte Clamp Thermal Perfusion Chamber 聞名於學界. 目前與 Harvard 公司合併, 及合併其他相產品類似的生技儀器公司, 已漸漸成為專業的大型儀器公司

美嘉儀器公司早在 Warner 成立之始, 即已與 Warner 建立深厚的合作關係迄今. 低價與實用的產品策略, 也深受研究界所推崇.

 

OC-725C Oocyte clamp amplifier and recording chamber RC-3Z Oocyte recording chamber
Planar Lipid Bilayer ( BLM ) Workstation

To record currents through actively gating, and, ion conducting single channels.

IE 251A / IE 210 Intracellular / Extracellular Amplifier / Electrometer.
Patch Clamp Amplifier ( PC505B / PC 501A ) Warner - Neurolog 電生理組合式放大器與分析軟體

Axon Pactch clamp 放大器與知名的 pCLAMP 分析軟體, 已是研究界所公認的最佳研究工具. 任何地方, 你都會發現所熟悉的儀器面板, 所熟悉的電腦分析軟體. 我們仍然爲您提供 Axon 設備, 也更樂於為您規劃膜電位紀錄與影像光譜的系統整合 ( , Infrared PatchClamp setup )

 

AxonPatch 200B AxonClamp 2B
DigiData 1322A pCLAMP 9.0
MultiClamp 700A 最新一代的微電腦操控放大器. 使用者可以在人性化的軟體介面上設定操作.
CyberAmp 380 / 320 多功能電生理紀錄系統 AxoGraph 4.0 多功能訊號擷取分析軟體

英國 OptoPatch - patch clamp 放大器, 在歐洲也頗具知名. 結合影像分析, 光譜分析, 提供研究另一扇窗. 其他產品包括螢光離子流影像分析系統, 光譜儀, 單光儀 ( Monochromator ). 結合電生理與光譜影像的應用系統, 最佳解決方法 ( Application solution for Electrophysiology and Optical Dunamic Imaging ).

IonWizard 軟體 ( Ionoptix Corp ) - 上圖

應用 : 動態 cell edge detection . 細胞生長長度量測. 個別 cardiac myocytes 收縮的長度變化量測  , 也可量測個別細胞的 sarcomere lengths .如果附加 I-CCD ( intensified camera) 積分式攝影器, 可供極弱螢光影像擷取.

 

 

高速影像擷取與光譜定量分析  

Fast Dynamic Optical Imaging & Data Acquisition, Analysis.

 

左圖 一: MDCK Cell loaded Fura 2 AM. 

左圖二 :

Fluo-3 labeled cells before and after stimulus.
 

高速影像擷取與光譜定量分析應用, 必須有完善的軟硬體規劃. 例如, 使用 Fura 2 及  Indo 1, 即是不一樣的軟硬體組配. 當然, 我們也必須是使用人員的樣品及螢光染劑, 以及, 實驗目的與經費範圍, 來一起綜合考量設備的規劃. 在完成規劃後, 我們也都會經由技術顧問作最後確認. 細心謹慎的事前作業, 目的只是爲客戶建立一套可以永續使用, 而且, 真正運作良好的系統.

美嘉公司是國內唯一有專業的技術團隊, 影像小組 ( Imaging Team )與 共軛焦小組 ( Confocal Team ). 小組所有成員接經過原廠多年的專業訓練, 每年也都經常赴國外接受技術與維修訓練. 兩個小組皆有 Leica 技術專員加入, 每年都來台指導我們與客戶. 此外,在國內我們也聘有多位博士學位的技術支援人員, 在技術顧問的指導下, 提供儀器教用與應用訓練, 也爲學校開課授課. 多年來, 我們已在各單位舉辦過近 50 場次的研習會.

我們很驕傲的說出, 我們的技術團隊, 遠遠優於國外. 在國內深根高偕影像技術, 是我們努力的目標

 

 

細胞與腦切片的紅外光影像與膜電位紀錄

Infrared Patch-Clamp Recording & Imaging 

 

 

複雜的系統組配, 對我們而言, 並不陌生. 我們從雜訊干擾的消除工程, 到有效訊號的結果取得, 皆已累積十幾年的經驗. 此類長時間累積出來的經驗, 正是使用人員最改安心與信任的技術提供者. 結合光譜影像與電生理系統, 絕對不是買一堆設備, 即可完成實驗. 讓我們來專注於儀器與技術, 讓您來專注科學的研究工作. ( 我們可為您架構顯微操作系統於任何廠牌任何型式的顯微鏡, 同時, 架構紅外光影像系統. 然而, 就光學解晰與顯微鏡性能而言, 我們還是建議你使用 DM LFSA. 因為 DM LFSA  完全無電場干擾, 六孔鏡頭鼻輪切換完全無震動干擾, 新一代 HCX APO L UVI 水鏡, 舉有長工作距離, 廣角設計, 耐酸鹼腐蝕的陶瓷法琅材質, 在紅外光具有高效的穿透率 , 完全微電腦智慧 Parfocal 設計, 鏡頭旋轉後, 鏡頭會自動浸入水溶液, 在上升再下降聚焦 , 此功能正是作 Patch-Clamp 工作最需要最方便的使用功能)

 

美嘉代理 Red-Shirt-Imaging 專業產品, 結合高速影像擷取與電生理紀錄系統, 提供心動態心肌細胞, 心臟, 神經, 神經細胞的光譜影像分析. 目前產品有 

 
心臟光譜影像擷取與分析軟體
神經光譜影像擷取與分析軟體
 
CAMERAS

Camera Chip* Well depth Array Pixel Area Chip Area Binning
Options
Max Readout speed Frame Transfer Time A/D bits**
SciMeasure CCD Cameras   (e-)   (µm2) (mm2)   (frames / sec) (µs)  
Cardio
CCD-SMQ
CCD39 215,000 80x80 24 1.92 x 1.92 1x1 2000 7 14
      40x40     2x2 3000    
      26x26     3x3 5000    
      80x12     1x1 10000    
  read noise#: 3e- at 40Hz, 4e- at 125Hz, 8e- at 1KHz, 12e- at 2KHz
 
Cardio
CCD-SM256
CCD67 600,000 256 x 256 26 6.66 x 6.66 1x1 100 322 14
    1,000,000 128x
128
    2x2 125    
      84x85     3x3 270    
      64x64     4x4 360    
  read noise#: 8e- at 7Hz, 11e- at 38Hz, 15e- at 62Hz, 28e- at 100Hz
* The e2v (Marconi) chips are back illuminated for maximun quantum yield (>80% at 400-600nm)
**A 16 bits options is available.


WuTech PhotoDiode Array
   
Cardio
PDA-III
H-468V >109 24x24 0.44 225## N/A 1600 N/A >18

##The fibers are organized in a hexagon 13 fibers to a side.

 

利用超高速 CCD 作光譜影像擷取., 應用 包括 :

 Intrinsic Signal Imaging
 Calcium Imaging
Potential Imaging

利用 Photodiode-Array 作為光譜影像擷取., 應用 包括 :

Population signals from intact brains and brain slices.
Multiple individual neurons in vertebrate and invertebrate ganglia.
Multiple sites on a single cell.
Tissue cultured neurons.
Heart cells.
Population signals from intact brains and brain slices.
Page display and multi-frame pseudocolor display of a control response (a) and epileptic activity (b) in brain slices. 
a) A control slice of piriform cortex stimulated in layer Ib at 400 mA (single trial). 
b) An epileptogenic slice stimulated in layer II at 275 mA (single trial). 
Each multi-frame display shows images at 3.8 msec intervals. Note the large secondary response in the epileptogenic slice.
In this experiment the site of onset was the dorsal edge of the En. Epileptiform activity which originated at this site spread to the overlying piriform cortex and the neighboring AI/PRha. 

-- Provided by Rezan Demir and Meyer Jackson, University of Wisconsin ( 摘錄至 RedShirtImaging 網站 )

Responses evoked by electrical stimulation of dentate gyrus in a hippocampal slice (frame interval = 2 msec)

-- Provided by Pavel Balaban ( 摘錄至 RedShirtImaging 網站 )

 

The Calcium indicator Rhod­2 was intra-cellularly injected with a patch pipette.
Ca transients induced by sodium action potentials were detected using a frame rate 2.7 kHz.

-- Provided by Hiroyoshi Miyakawa, Masashi Inoue and Yoshihisa Kudo, Laboratory of Cellular Neurobiology, Tokyo University of Pharmacy and Life Science ( 摘錄至 RedShirtImaging 網站 )

 

Action potential in a hippocampus CA1 pyramidal neuron detected with a voltage-sensitive-dye (JPW1114).

-- Provided by Hiroyoshi Miyakawa, Masashi Inoue and Yoshihisa Kudo, Laboratory of Cellular Neurobiology, Tokyo University of Pharmacy and Life Science

 

摘錄相關應用文獻 ( references ) :

Ying-wan Lam, Lawrence B. Cohen  and Michal R. Zochowski (2003) Odorant specificity of three oscillations and the DC signal in the turtle olfactory bulb. Eur. J. Neurosci. Feb;17(3):436-446

Scharfman HE, Sollas AL, Smith KL, Jackson MB, Goodman JH. (2002) Structural and functional asymmetry in the normal and epileptic rat dentate gyrus. J Comp Neurol Dec;454(4):424-39

Demir R, Gao BX, Jackson MB, Ziskind-Conhaim L. (2002) Interactions between multiple rhythm generators produce complex patterns of oscillation in the developing rat spinal cord.  J Neurophysiol. Feb;87(2):1094-105

Jin W, Zhang RJ, Wu J. (2002) Voltage-sensitive dye imaging of population neuronal activity in cortical tissue. J Neurosci Methods 115(1):13-27

Laaris N, Keller A. (2002) Functional independence of layer IV barrels. J. Neurophysiol Feb;87(2):1028-34

R. Köhling , a, J. Reinelb, J. Vahrenholdb, K. Hinrichsb and E. -J. Speckmanna (2002) Spatio-temporal patterns of neuronal activity: analysis of optical imaging data using geometric shape matching.  J Neurosci. Methods. Feb 15;114(1):17-23.

Schemann M, Michel K, Peters S, Bischoff SC, Neunlist M. (2002) Cutting-edge technology. III. Imaging and the gastrointestinal tract: mapping the human enteric nervous system. Am J Physiol Gastrointest Liver Physiol. 2002 Jun;282(6):G919-25

Wang C, Kang-Park MH, Wilson WA, Moore SD (2002) Properties of the pathways from the lateral amygdal nucleus to basolateral nucleus and amygdalostriatal transition area. J Neurophysiol 87(5):2593-601.

Demir R, Haberly LB, Jackson MB (2001) Epileptiform discharges with in-vivo-like features in slices of rat piriform cortex with longitudinal association fibers. J Neurophysiol. 86(5):2445-60.

Hiraku Mochida; Katsushige Sato; Yoshiyasu Arai; Shinichi Sasaki; Itaru Yazawa; Kohtaro Kamino; Yoko Momose-Sato (2001) Multiple-site optical recording reveals embryonic organization of synaptic networks in the chick spinal cord. European Journal of Neuroscience 13(8); 1547

Mao,B.-Q., Hamzei-Sichani F, Aronov D, Froemke R.C., Yuste R. (2001) Dynamics of Spontaneous Activity in Neocortical slices. Neuron 32:883-898.

Mochida H, Sato K, Arai Y, Sasaki S, Kamino K, Momose-Sato Y (2001) Optical imaging of spreading depolarization waves triggered by spinal nerve stimulation in the chick embryo: possible mechanisms for large-scale coactivation of the central nervous system. Eur J Neurosci 14(5):809-20.

Mochida H, Sato K, Sasaki S, Yazawa I, Kamino K, Momose-Sato Y ( 2001) Effects of anisomycin on LTP in the hippocampal CA1: long-term analysis using optical recording. Neuroreport. 17;12(5):987-91.

Momose-Sato Y, Sato K, Kamino K. (2001) Optical approaches to embryonic development of neural functions in the brainstem. Prog Neurobiol. 63(2):151-97. Review.

Momose-Sato Y, Sato K, Mochida H, Yazawa I, Sasaki S, Kamino K. (2001) Spreading depolarization waves triggered by vagal stimulation in the embryonic chick brain: optical evidence for intercellular communication in the developing central nervous system. Neuroscience. 102(2):245-262.

Sato K, Mochida H, Sasaki S, Yazawa I, Kamino K, Momose-Sato Y. (2001) Optical responses to micro-application of GABA agonists in the embryonic chick brain stem. Neuroreport. 22;12(1):95-8.

Wachowiak M, Cohen LB (2001). Representation of odorants by receptor neuron input to the mouse olfactory bulb. Neuron 32(4):723-35

Wang C, Wilson WA, Moore SD.  (2001) Role of NMDA, Non-NMDA, and GABA Receptors in Signal Propagation in the Amygdala Formation  J Neurophysiol. Sep;86(3):1422-9

Wu JY, Guan L, Bai L, Yang Q. (2001) Spatiotemporal Properties of an Evoked Population Activity in Rat Sensory Cortical Slices. J Neurophysiol. 86(5):2461-74

Antic S, Wuskell JP, Loew L, Zecevic D (2000) Functional profile of the giant metacerebral neuron of helix aspersa: temporal and spatial dynamics of electrical activity in situ. J Physiol. 527 (1):55-69.

Demir R, Haberly LB, Jackson MB (2000) Imaging epileptiform discharges in slices of piriform cortex with voltage-sensitive fluorescent dyes. Ann N Y Acad Sci. 911:404-17.

Demir R, Haberly LB, Jackson MB (2000) Characteristics of plateau activity during the latent period prior to epileptiform discharges in slices from rat piriform cortex. J Neurophysiol. 83(2):1088-98.

Kohling R, Hohling JM, Straub H, Kuhlmann D, Kuhnt U, Tuxhorn I, Ebner A, Wolf P, Pannek HW, Gorji A, Speckmann EJ. (2000) Optical monitoring of neuronal activity during spontaneous sharp waves in chronically epileptic human neocortical tissue. J Neurophysiol. 84(4):2161-5.

Laaris, Nora , Carlson, Greg C. and Keller, Asaf (2000). Thalamic-Evoked Synaptic Interactions in Barrel Cortex Revealed by Optical Imaging. J. Neurosci. 20(4):1529-37.

Ying-wan Lam, Lawrence B. Cohen, Matt Wachowiak, and Michal R. Zochowski (2000). Odors elicit three different oscillations in the turtle olfactory bulb. J. Neurosci. 20(2):749-762.

Peterlin ZA, Kozloski J, Mao BQ, Tsiola A, Yuste R (2000) Optical probing of neuronal circuits with calcium indicators. Proc Natl Acad Sci USA 97(7):3619-24.

Muschol M, Salzberg BM (2000) Dependence of transient and residual calcium dynamics on action-potential patterning during neuropeptide secretion. J Neurosci. 20(18):6773-80.

Sato K, Yazawa I, Mochida H, Sasaki S, Kamino K, Momose-Sato Y. (2000) Optical detection of embryogenetic expression of vagal excitability in the rat brain stem. Neuroreport 11(17):3759-63.

Tanaka T, Yazawa I., Sato K., Momose-Sato Y. and Kamino K (2000) Consistency behind trial-to-trial variation in intrinsic optical responses to single-whisker movement in the rat D1-barrel cortex. Neurosci Res. 36(3):193-207.

Wachowiak M, Zochowski M, Cohen LB, Falk CX. (2000) The spatial representation of odors by olfactory receptor neuron input to the olfactory bulb is concentration invariant. Biol Bull. 199(2):162-3.

Zochowski M, Cohen LB, Fuhrmann G, Kleinfeld D. (2000 ) Distributed and partially separate pools of neurons are correlated with two different components of the gill-withdrawal reflex in Aplysia. J Neurosci. 20(22):8485-92.

Zochowski M, Wachowiak M, Falk CX, Cohen LB, Lam YW, Antic S, Zecevic D. (2000). Imaging membrane potential with voltage-sensitive dyes. Biol Bull. 198(1):1-21. Review.

Antic S, Major G, Zecevic D (1999). Fast optical recordings of membrane potential changes from dendrites of pyramidal neurons. J Neurophysiol. 82(3):1615-21.

Arai Y, Momose-Sato Y, Sato K, Kamino K (1999). Optical mapping of neural network activity in chick spinal cord at an intermediate stage of embryonic development. J Neurophysiol. 81(4):1889-902.

Demir R, Haberly LB, Jackson MB (1999) Sustained plateau activity precedes and can generate ictal-like discharges in low-Cl(-) medium in slices from rat piriform cortex. J Neurosci. 19(24):10738-46.

Demir R, Haberly LB, Jackson MB (1999). Sustained and accelerating activity at two discrete sites generate epileptiform discharges in slices of piriform cortex. J Neurosci. 19(4):1294-306

Kubota M, Nasu M, Taniguchi I (1999) Layer-specific horizontal propagation of excitation in the auditory cortex. Neuroreport 10(13):2865-7

Momose-Sato Y, Sato K, Arai Y, Yazawa I, Mochida H, Kamino K (1999) . Evaluation of Voltage-Sensitive Dyes for Long-Term Recording of Neural Activity in the Hippocampus. J Membr Biol. 172(2):145-157.

Momose-Sato Y, Sato K, Kamino K (1999). Optical identification of calcium-dependent action potentials transiently expressed in the embryonic rat brainstem. Neuroscience. 90(4):1293-310.

Momose-Sato Y, Komuro H, Hirota A, Sakai T, Sato K, Kamino K (1999). Optical imaging of the spatiotemporal patterning of neural responses in the embryonic chick superior cervical ganglion. Neuroscience. 90(3):1069-83

Neunlist M, Peters S, Schemann M (1999). Multisite optical recording of excitability in the enteric nervous system. Neurogastroenterol Motil. 11(5):393-402.

Obaid AL, Koyano T, Lindstrom J, Sakai T, Salzberg BM (1999). Spatiotemporal patterns of activity in an intact mammalian network with single-cell resolution: optical studies of nicotinic activity in an enteric plexus. J Neurosci. 19(8):3073-93.

Sato K, Momose-Sato Y, Mochida H, Arai Y, Yazawa I, Kamino K (1999). Optical mapping reveals the functional organization of the trigeminal nuclei in the chick embryo. Neurosci. 93(2):687-702.

Senseman DM, Robbins KA (1999). Modal behavior of cortical neural networks during visual processing. J Neurosci. 15;19(10):RC3 .

Senseman DM (1999). Spatiotemporal structure of depolarization spread in cortical pyramidal cell populations evoked by diffuse retinal light flashes. Vis Neurosci. 16(1):65-79.

Tsau Y, Guan L, Wu JY (1999). Epileptiform Activity Can Be Initiated in Various Neocortical Layers: An Optical Imaging Study. J Neurophysiol. 82(4):1965-1973.

Wu JY, Guan L, Tsau Y (1999). Propagating activation during oscillations and evoked responses in neocortical slices. J Neurosci. 19(12):5005-15.

Wachowiak M, Cohen LB (1999). Presynaptic inhibition of primary olfactory afferents mediated by different mechanisms in lobster and turtle. J Neurosci. 19(20):8808-17.

Albowitz B, Kuhnt U, Kohling R, Lucke A, Straub H, Speckmann EJ, Tuxhorn I, Wolf P, Pannek H, Oppel F (1998). Spatio-temporal distribution of epileptiform activity in slices from human neocortex: recordings with voltage-sensitive dyes. Epilepsy Res. 32(1-2):224-32

Demir R, Haberly LB, Jackson MB (1998). Voltage imaging of epileptiform activity in slices from rat piriform cortex: onset and propagation. J Neurophysiol. 80(5):2727-42 .

Keller A, Yagodin S, Aroniadou-Anderjaska V, Zimmer LA, Ennis M, Sheppard NF Jr, Shipley MT (1998). Functional organization of rat olfactory bulb glomeruli revealed by optical imaging. J Neurosci. 18(7):2602-12.

Momose-Sato Y, Sato K, Hirota A, Kamino K (1998). GABA-Induced intrinsic light-scattering changes associated with voltage-sensitive dye signals in embryonic brain stem slices: coupling of depolarization and cell shrinkage. J Neurophysiol. 79(4):2208-17

Sakai T, Momose-Sato Y, Sato K, Hirota A, Kamino K (1998). Optical monitoring of synaptic transmission in bullfrog sympathetic ganglia using a voltage-sensitive dye. Neurosci Lett. 242(1):1-4.

Sakai T, Yada T, Hirota A, Komuro H, Kamino K (1998). A regional gradient of cardiac intrinsic rhythmicity depicted in embryonic cultured multiple hearts. Pflugers Arch. 437(1):61-9.

Sato K, Momose-Sato Y, Hirota A, Sakai T, Kamino K (1998). Optical mapping of neural responses in the embryonic rat brainstem with reference to the early functional organization of vagal nuclei. J Neurosci. 18(4):1345-62.

Wachowiak M, Cohen LB (1998).Presynaptic afferent inhibition of lobster olfactory receptor cells: reduced action-potential propagation into axon terminals. J Neurophysiol. 80(2):1011-5

Wu JY, Lam YW, Falk CX, Cohen LB, Fang J, Loew L, Prechtl JC, Kleinfeld D, Tsau Y (1998). Voltage-sensitive dyes for monitoring multineuronal activity in the intact central nervous system. Histochem J. 30(3):169-87.

Zecevic D, Antic S (1998). Fast optical measurement of membrane potential changes at multiple sites on an individual nerve cell. Histochem J. 1998 Mar;30(3):197-216.

Antic S, Major G, Chen WR, Wuskel J, Loew L, Zecevic D (1997). Fast voltage-sensitive dye recording of membrane potential changes at multiple sites on an individual nerve cell in the rat cortical slice. Biol Bull. 193(2):261.

Albowitz B, Konig P, Kuhnt U (1997). Spatiotemporal distribution of intracellular calcium transients during epileptiform activity in guinea pig hippocampal slices. J Neurophysiol. 77(1):491-501.

Hickie C, Cohen LB, Balaban PM (1997). The synapse between LE sensory neurons and gill motoneurons makes only a small contribution to the Aplysia gill-withdrawal reflex. Eur J Neurosci. 9(4):627-36

Prechtl, J.C., Cohen L.B., Peseran B., Mitra P.P., and Kleinfeld D. (1997). Visual stimuli induce waves of electrical activity in turtle cortex. Proc Nat Acad Sci (USA), 94:7621-7626.

Momose-Sato Y, Sato K, Hirota A, Sakai T, Yang XS, Kamino K (1997). Optical characterization of a novel GABA response in early embryonic chick brainstem. Neuroscience. 80(1):203-19.

Sakai T, Hirota A, Momose-Sato Y, Sato K, Kamino K (1997). Optical mapping of conduction patterns of normal and tachycardia-like excitations in the rat atrium. Jpn J Physiol. 47(2):179-88.

Sato K, Momose-Sato Y, Arai Y, Hirota A, Kamino K (1997). Optical illustration of glutamate-induced cell swelling coupled with membrane depolarization in embryonic brain stem slices. Neuroreport. 8(16):3559-63.

Hopp HP, Falk CX, Cohen LB, Wu JY, Cohen AI (1996). Effect of feedback from peripheral movements on neuron activity in the aplysia abdominal ganglion. Eur J Neurosci. 8(9):1865-72

Jackson, M.B. and Scharfman H.E. (1997). Positive feedback from hilar mossy cells to granule cells in the dendate gyrus revealed by voltage-sensitive dye and microelectrode recording. J. Neurophysiology, 76:601-616.

Langenstroth M, Albowitz B, Kuhnt U (1996). Partial suppression of GABAA-mediated inhibition induces spatially restricted epileptiform activity in guinea pig neocortical slices. Neurosci Lett. 210(2):103-6.

Obaid A.L. and Salzberg B.M. (1996). Micromolar 4­aminopyridine enhances invasion of a vertebrate neurosecretory terminal arborization: optical recording of action potential propagation using an ultrafast photodiode­MOSFET camera and a photodiode array. J. Gen. Physiology, 107:353­368.

Sato K, Momose-Sato Y, Hirota A, Sakai T, Kamino K (1996). Optical studies of the biphasic modulatory effects of glycine on excitatory postsynaptic potentials in the chick brainstem and their embryogenesis. Neuroscience. 72(3):833-46.

Senseman D.M. (1996). Correspondence between visually evoked voltage­sensitive dye signals and synaptic activity recorded in cortical pyramidal cells with intracellular microelectrodes. Vis Neuroscience, 13:963­977.

Senseman D.M. (1996). High­speed optical imaging of afferent flow through rat olfactory bulb slices: voltage­sensitive dye signals reveal periglomerular cell activity. J. Neuroscience, 16(1):313-324.

Tsau, Y., P. Wenner, M.J. O'Donovan, L.B. Cohen, L.M. Loew, and J.P. Wuskell. (1996). Dye screening and signal-to-noise ratio for retrogradely transported voltage-sensitive dyes. J Neurosci Methods, 70(2):121-129.

Wenner, P., Y. Tsau, L.B. Cohen, M.J. O'Donovan, and Y. Dan. (1996). Voltage-sensitive dye recording using retrogradely transported dye in the chicken spinal cord: staining and signal characteristics. J Neurosci Methods, 70(2):111-120.

Zecevic, D. (1996). Multiple spike-initiation zones in single neuron revealed by voltage-sensitive dyes. Nature, 381:322-325.

Antic, S. and Zecevic D. (1995). Optical signals from neurons with internally applied voltage-sensitive dyes. J. Neuroscience, 15:1392-1405.

Albowitz B, Kuhnt U (1995). Epileptiform activity in the guinea-pig neocortical slice spreads preferentially along supragranular layers--recordings with voltage-sensitive dyes. Eur J Neurosci. 7(6):1273-84

Kogan A, Ross WN, Zecevic D, Lasser-Ross N (1995). Optical recording from cerebellar Purkinje cells using intracellularly injected voltage-sensitive dyes. Brain Res. 700(1-2):235-9 .

Momose-Sato Y, Sato K, Sakai T, Hirota A, Kamino K (1995). A novel gamma-aminobutyric acid response in the embryonic brainstem as revealed by voltage-sensitive dye recording. Neurosci Lett.191(3):193-6.

Momose-Sato Y, Sato K, Sakai T, Hirota A, Matsutani K, Kamino K (1995). Evaluation of optimal voltage-sensitive dyes for optical monitoring of embryonic neural activity. J Membr Biol 144(2):167-76.

Sato K, Momose-Sato Y, Sakai T, Hirota A, Kamino K (1995).Responses to glossopharyngeal stimulus in the early embryonic chick brainstem: spatiotemporal patterns in three dimensions from repeated multiple-site optical recording of electrical activity. J Neurosci. 15(3 Pt 2):2123-40

Demir, R., Haberly L. B., and Jackson M. (1994). Optical recordings of evoked and spontaneous epileptiform discharges and slices from piriform cortex. Soc. Neurosci. Abstr. 20:403.

Momose-Sato Y, Sakai T, Hirota A, Sato K, Kamino K (1994). Optical mapping of early embryonic expressions of Mg(2+)-/APV-sensitive components of vagal glutaminergic EPSPs in the chick brainstem. J Neurosci. 14(12):7572-84.

Rohr, S. and Salzberg, B.M. (1994). Multiple site optical recording of transmembrane voltage (MSORTV) in patterned growth heart cell cultures: assessing electrical behavior, with microsecond resolution, on a cellular and subcellular scale. Biophysical Journal, 67:1301-1315.

Rohr S, Salzberg BM (1994). Characterization of impulse propagation at the microscopic level across geometrically defined expansions of excitable tissue: multiple site optical recording of transmembrane voltage (MSORTV) in patterned growth heart cell cultures. J Gen Physiol. 104(2):287-309.

Senseman D.M. and Rea M.A. (1994). Fast multisite optical recording of mono­ and polysynaptic activity in the hamster suprachiasmatic nucleus evoked by retinohypothalamic tract stimulation. Neuroimage, 1:247­263.

Wu, J-Y., Cohen L.B., Falk C.X. (1994). Neuronal activity during different behaviors in Aplysia: A distributes organization? Science, 263:820-823.

Falk, C.X., Wu J-Y., Cohen L.B. and Tang A.C. (1993). Nonuniform expression of habituation in the activity of distinct classes of neurons in the Aplysia abdominal ganglion. J Neuroscience, 13:4072-4081.

Komuro H, Momose­Sato Y, Sakai T, Hirota A, Kamino K. (1993). Optical monitoring of early appearance of spontaneous membrane potential changes in the embryonic chick medulla oblongata using a voltage­sensitive dye. Neuroscience, 52(1):55-62.

Salzberg BM, Obaid AL, Bezanilla F (1993). Microsecond response of a voltage-sensitive merocyanine dye: fast voltage-clamp measurements on squid giant axon. Jpn J Physiol. 43 Suppl 1:S37-41.

Cinelli A.R. and Salzberg B.M. (1992). Dendritic origin of late events in optical recordings from salamander olfactory bulb. J. Neurophysiology, 68:786­806.

Nakashima, M., S. Yamada, S. Shiono, M. Maeda, and F. Sato. (1992). 448­detector optical recording system: development and application to Aplysia gill­withdrawalreflex. IEEE Trans. on Biomedical Engineering, 39(1):26­36.

Parsons T.D., Obaid A.L. and Salzberg B.M. (1992). Aminoglycoside antibiotics block voltage­dependent calcium channels in intact vertebrate nerve terminals. J. Gen. Physiology, 99:491­504.

Parsons T.D., Salzberg B.M., Obaid A.L., Raccuia-Behling F. and Kleinfeld D. (1991). Long-term optical recording of patterns of electrical activity in ensembles of cultured Aplysia neurons. J. Neurophysiology, 66(1):316-333.

 

 

活細胞紀錄應用之共軛焦與多光子影像系統 

Confocal & Multi-Photon Imaging System 

 

左圖 : 採用 Leica TCS SP 共軛焦光譜顯微鏡 ( 配合 Ar-Kr laser AOTF ), Leica DM LFSA 顯微鏡上 ( 採用新一代 HCX APO L 水鏡 ), 架設 Luigs Neumann 的顯微操作系統附加 IR-CCD Camera 作影像擷取. 此外, 同時作電生理膜電位紀錄 ( Pacth clamp ). - 多光子雷射提供活細胞影像擷取應用. ( TCS SP2-RS 則為 Leica 新一代高速掃描的共軛焦系統. )
美嘉儀器股份有限公司 Major Instruments Co., Ltd.
台北總公司 : TEL : 02-2808-1452  FAX : 02-2808-2354  維修中心 : 02-2808-2353   高雄辦事處 - TEL : (07) 725-8177  FAX : (07) 725-8204

E-mail : major@major.com.tw    http://www.major.com.tw