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P-2000 拉針儀

Sutter P-2000 為利用氣體雷射做為拉針熱源, 可應用於玻璃與石英光纖微管制作.







COMMON APPLICATIONS P-2000/G
  • Patch clamp - single isolated
  • and whole cell
  • Intracellular recording
  • Microinjection
  • Nano probe research
  • SECM
 
P-2000/F
  • Nanospray mass spectrometry
  • NSOM
  • Tapering optical fibers
 
FEATURES
  • Capable of pulling quartz, borosilicate and aluminosilicate glass.
  • Fully programmable - including heating filament characteristics.
  • The laser has no melting point limit as with conventional metal filaments; and therefore, cannot be burned out.
  • Pulls electrodes with tip diameters smaller than 0.03µm.
  • Optimized velocity sensing circuit for maximized sensitivity and reproducibility.*
  • Operating life of the CO2 laser is expected to be in excess of ten years with normal use, after which the laser can be refurbished by the Sutter Instrument Company for a nominal charge.
  • Individual programs can be write-protected in order to secure them from inadvertent changes.
  • The total time that the heat is on during the pull is displayed for improved program development and troubleshooting.
  • A date and time stamp is displayed to show the last time that a program has been changed.
  • The P-2000/F is ideal for applications such as nanospray and NSOM.
  • Preprogrammed sample programs for intra-cellular and patch pipettes. The P-2000/F also comes with an NSOM tip program. 
 
REFERENCES
These references describe the Flaming/Brown pullers and contain valuable information applicable to the P-2000.

1 Munoz, J.L. and Coles, J. Quartz micropipettes for intracellular voltage micro- electrodes and ion selective microelectrodes. Journal of Neuroscience Methods: 22:57-64, 1987.

2 Rae, J.L. and Levis, R. A. A method for exceptionally low noise single channel recordings. European Journal of Physiology - Pflügers Archiv: 420:618-620, 1992.

3 Zuazaga, C. and Steinacker, A. Patch-clamp recording of ion channels: Interfering effects of patch pipette glass. News in Physiological Science: 5:155-159, 1990.

4 Levis, R.A. and Rae, J. L. The use of quartz patch pipettes for low noise single channel recording. Biophysical Journal: 65:1666-1677, 1993.

 
 

USER MANUALS
P-2000 Operation Manual

PRODUCT INFORMATION
TECHNOTES: Which P-2000 model do I need?
TECHNOTES: Novel applications for P-2000

VIDEO TUTORIALS:
P-2000 Introduction
Pulling a Patch Pipette From Quartz Glass
Effects of The Velocity Parameter
Copying Pull Programs on the P-2000 Puller
Troubleshooting Pipette Variablity
Puller Bars and V-groove Bearings
Checking the Pull Cables
Checking the Cable Pulleys
WEBINAR: General Maintenance