DMi8 S Module Infinite TIRF

Leica Infinity TIRF  光路設計 與 TIRF 技術原理

TIRF (Total Internal Reflection Fluorescence) 利用雷射光入射角度達到全反射時，在介質表面形成的漸逝波（Evanescent Wave）做為激發螢光的能量來源，由於漸逝波的深度僅於表面 70~300 nm，較深的螢光物質便不會被激發，只有極靠近全反射面的樣本區域會產生螢光反射，大大降低了背景光雜訊干擾觀測標的，故此項技術廣泛應用於細胞表面物質的動態觀察。因此可觀察表面螢光表現而不受干擾 ( 例如細胞膜內外的動態觀察 )，為提高 Z 軸解析度之一項技術.

全內反射螢光顯微術 ( TIRFM) 是探索細胞基底的淺層接觸區域內的細胞生命活動現象的有效光學探測方法.，如細胞膜內蛋白質的動力學過程，基底附近的細胞骨架，細胞運動…等, 可應用於即時觀察單一肌漿球蛋白分子的運動、單一蛋白分子對之間的螢光共振能量轉移 (FRET),…..酶的轉譯，聚合物內單個分子的結構變化, 等離子體膜附近的神經分泌腺的顆粒運動.








1 TIRF Sensor: Detects the back reflected beam and enabling reproducible automated adjustment of TIRF penetration depth in every sample

2 TIRF Scanner: Fine-tuning of TIRF penetration depth and adjustment of illumination direction (azimuth)

3 Movable collimator: Allows TIRF over full z-travel range with all Leica TIRF objectives

4 Merge Optics: Combines a second illumination light path through Infinity TIRF module

5 Beam Expander: Infinity TIRF HP module increases the power density on the sample for super-resolution imaging





無疑的, LEICA Infinity TIRF 的推出, 在科研領域, 已造成震撼. 因為 LEICA Infinity TIRF 的創新設計, 可以創造使用者的最大價值, 讓使用者得到從所未有的超高解析的全反射螢光影像. 讓使用者得以探索細胞分子奧秘. 讓所有競爭廠商的設計, 汗顏落後. 因為 LEICA Infinity TIRF 的優越設計, 我們膽敢驕傲的說出, 這是全世界最好的全反射螢光顯微鏡系統

LEICA 獨家設計的 智慧型 Infinity TIRF Scanner 可精確自動調整雷射光 ( multi-color laser ) 入射角度，得到高解析度 TIRF 影像更為簡易。使用一般手動 TIRF 時，耗時費力調整卻無法得到正確入射角度，更換樣本後，又必須再次調整雷射角度。使用全自動 LEICA Infinity TIRF , 只要輕鬆按下滑鼠，便可以自動校正雷射光全反射角度，並可選擇四種不同的雷射入射方向，不再只能得到單一方向的 TIRF 影像，領先突破現有的研究範疇！

因為 LEICA Infinity TIRF 可以簡易的得到超高解析的全反射螢光現象, 讓你專注於樣品研究, 而不是浪費時間在調整雷射或光徑角度.   LEICA Infinity TIRF 的獨家設計, 雷射光入射角度是全自動調整,  並可精確的控制 全反射的漸逝波深度 ( evanescent wave ), 可讓你一層一層觀察全反射螢光斷面影像, 可經確的探討細胞膜內外的動態螢光影像, 突破極俱研究價值的使用效益. 

因為控制全反射漸逝波（evanescent wave）深度，不同雷射波長，也可保持固定的漸逝波深度，更能增加實驗結果的可信度及再現性。漸逝波深度經由軟體可自由調整，得到不同漸逝波深度下的全反射螢光影像，提供了更多可能性的研究方向
 









Hela cells stably expressing Actin Chromobody-Tag GFP2 and stained with SIR-Tubulin. Courtesy of ChromoTek GmbH, Munich, Germany, and Spirochrome SA.



dSTORM image of microtubules stained with Alexa 647

Super-resolution

Supercharge your system with super-resolution and nanoscopy capabilities. Choose the Infinity TIRF High Power (HP) system to give you the ability to achieve multi-color single molecule resolution at the cell surface to resolve structures involved in receptor trafficking, synaptic organization, or cell adhesion.

With the high-powered lasers option, you can image and analyze down to 20nm resolution with techniques such as single molecule particle tracking, GSD, dSTORM and uPaint.


 

Publications 2019

Spatial control of the GTPase MglA by localized RomR–RomX GEF and MglB GAP activities enables Myxococcus xanthus motility
Szadkowski D, Harms A, Carreira L, Wigbers M, Potapova A, et. al.
Nature Microbiology
2019 vol: 4 (8) pp: 1344-1355

Membrane-cytoskeletal crosstalk mediated by myosin-I regulates adhesion turnover during phagocytosis
Barger S, Reilly N, Shutova M, Li Q, Maiuri P, et. al.
Nature Communications
2019 vol: 10 (1) pp: 1249

TFEB controls vascular development by regulating the proliferation of endothelial cells
Doronzo G, Astanina E, Corà D, Chiabotto G, Comunanza V, et. al.
The EMBO Journal
2019 vol: 38 (3)

Publications 2018

DNA Nanostructure-Programmed Like-Charge Attraction at the Cell-Membrane Interface
Ding H, Li J, Chen N, Hu X, Yang X, et. al.
ACS Central Science
2018 vol: 4 (10) pp: 1344-1351

Polymicrobial sepsis influences NK-cell-mediated immunity by diminishing NK-cell-intrinsic receptor-mediated effector responses to viral ligands or infections
Jensen I, Winborn C, Fosdick M, Shao P, Tremblay M, et. al.
PLOS Pathogens
2018 vol: 14 (10) pp: e1007405

Deciphering Virus Entry with Fluorescently Labeled Viral Particles
Hoffmann A, Mazelier M, Léger P, Lozach P
Publisher: Humana Press, New York, NY
2018 pp: 159-183

Priming of dendritic cells by DNA-containing extracellular vesicles from activated T cells through antigen-driven contacts
Torralba D, Baixauli F, Villarroya-Beltri C, Fernández-Delgado I, Latorre-Pellicer A, et. al.
Nature Communications
2018 vol: 9 (1) pp: 2658

The effect of a solvent on cellular response to PCL/gelatin and PCL/collagen electrospun nanofibres
Dulnik J, Kołbuk D, Denis P, Sajkiewicz P
European Polymer Journal
2018 vol: 104 pp: 147-156

Glycerol monolaurate induces filopodia formation by disrupting the association between LAT and SLP-76 microclusters
Zhang M, Tran P, Wolff A, Tremblay M, Fosdick M, Houtman J
Science Signaling
2018 vol: 11 (528) pp: eaam9095

Separating Actin-Dependent Chemokine Receptor Nanoclustering from Dimerization Indicates a Role for Clustering in CXCR4 Signaling and Function
Martínez-Muñoz L, Rodríguez-Frade J, Barroso R, Sorzano C, Torreño-Pina J, et. al.
Molecular Cell
2018 vol: 70 (1) pp: 106-119.e10

Sema4C/PlexinB2 signaling controls breast cancer cell growth, hormonal dependence and tumorigenic potential
Gurrapu S, Pupo E, Franzolin G, Lanzetti L, Tamagnone L
Cell Death & Differentiation
2018 pp: 1

β-catenin is important for the development of an insulin responsive pool of GLUT4 glucose transporters in 3T3-L1 adipocytes
Dissanayake W, Sorrenson B, Cognard E, Hughes W, Shepherd P
Experimental Cell Research
2018 vol: 366 (1) pp: 49-54

Tbx5a lineage tracing shows cardiomyocyte plasticity during zebrafish heart regeneration
Sánchez-Iranzo H, Galardi-Castilla M, Minguillón C, Sanz-Morejón A, González-Rosa J, et. al.
Nature Communications
2018 vol: 9 (1) pp: 428

Publications 2017

Pro-necrotic Activity of Cationic Mastoparan Peptides in Human Glioblastoma Multiforme Cells Via Membranolytic Action
da Silva A, Silva-Gonçalves L, Oliveira F, Arcisio-Miranda M
Molecular Neurobiology
2017 pp: 1-15

L-DOPA sensitizes vasomotor tone by modulating the vascular alpha1-adrenergic receptor
Masukawa D, Koga M, Sezaki A, Nakao Y, Kamikubo Y, et. al.
JCI Insight
2017 vol: 2 (18)

Negative regulation of urokinase receptor activity by a GPI-specific phospholipase C in breast cancer cells
van Veen M, Matas-Rico E, van de Wetering K, Leyton-Puig D, Kedziora K, et. al.
eLife
2017 vol: 6 pp: e23649

Assembly, Secretory Pathway Trafficking, and Surface Delivery of Kainate Receptors Is Regulated by Neuronal Activity
Evans A, Gurung S, Wilkinson K, Stephens D, Henley J
Cell Reports
2017 vol: 19 (12) pp: 2613-2626

Nanodiamonds Mediate Oral Delivery of Proteins for Stem Cell Activation and Intestinal Remodeling in Drosophila
Hu X, Li X, Yin M, Li P, Huang P, et. al.
ACS Applied Materials & Interfaces
2017 pp: acsami.7b04788

eNOS S-nitrosylates β-actin on Cys374 and regulates PKC-θ at the immune synapse by impairing actin binding to profilin-1
García-Ortiz A, Martín-Cofreces N, Ibiza S, Ortega Á, Izquierdo-Álvarez A, et. al.
PLOS Biology
2017 vol: 15 (4) pp: e2000653

In situ self-assembly of gold nanoparticles on hydrophilic and hydrophobic substrates for influenza virus-sensing platform
Ahmed S, Kim J, Tran V, Suzuki T, Neethirajan S, et. al.
Scientific Reports
2017 vol: 7 pp: 44495

Dual-agonist occupancy of orexin receptor 1 and cholecystokinin A receptor heterodimers decreases G-protein–dependent signaling and migration in the human colon cancer cell line HT-29
Bai B, Chen X, Zhang R, Wang X, Jiang Y, et. al.
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
2017 vol: 1864 (7) pp: 1153-1164

Analysis of Microtubules and Microtubule-Organizing Center at the Immune Synapse
Blas-Rus N, Bustos-Morán E, Sánchez-Madrid F, Martín-Cófreces N
2017 pp: 31-49

Apelin receptor homodimer-oligomers revealed by single-molecule imaging and novel G protein-dependent signaling
Cai X, Bai B, Zhang R, Wang C, Chen J
Scientific Reports
2017 vol: 7 pp: 40335

Publications 2016

 

PPFIA1 drives active α5β1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis
Mana G, Clapero F, Panieri E, Panero V, et.al.
Nature Communications
2016 vol: 7 pp: 13546

Highly deformable nanofilaments in flow
Pawłowska S
Journal of Physics: Conference Series
2016 vol: 760 pp: 012022

Developmental epigenetic programming of adult germ cell death disease: Polycomb protein EZH2–miR-101 pathway
Siddeek B, Lakhdari N, Inoubli L, Paul-Bellon R, Isnard V, et. al.
Epigenomics
2016 vol: 8 (11) pp: 1459-1479

Human Serum Albumin (HSA) Suppresses the Effects of Glycerol Monolaurate (GML) on Human T Cell Activation and Function
Zhang M, Houtman J
PLOS ONE
2016 vol: 11 (10) pp: e0165083

Thiol dependent intramolecular locking of Orai1 channels
Alansary D, Schmidt B, Dörr K, Bogeski I, Rieger H, et. al.
Scientific Reports
2016 vol: 6 pp: 33347

The myosin X motor is optimized for movement on actin bundles
Ropars V, Yang Z, Isabet T, Blanc F, Zhou K, et. al.
Nature Communications
2016 vol: 7 pp: 12456

Diffusion Tensor Analysis by Two-Dimensional Pair Correlation of Fluorescence Fluctuations in Cells
Di Rienzo C, Cardarelli F, Di Luca M, Beltram F, Gratton E
Biophysical Journal
2016 vol: 111 (4) pp: 841-851

Glycerol Monolaurate (GML) inhibits human T cell signaling and function by disrupting lipid dynamics
Zhang M, Sandouk A, Houtman J, Schlievert P, Deringer J, et. al.
Scientific Reports
2016 vol: 6 pp: 30225

Expression of Nucleolin Affects Microtubule Dynamics
Gaume X, Place C, Delage H, Mongelard F, Monier K, et. al.
PLOS ONE
2016 vol: 11 (6) pp: e0157534

RAB2A controls MT1-MMP endocytic and E-cadherin polarized Golgi trafficking to promote invasive breast cancer programs
Kajiho H, Kajiho Y, Frittoli E, Confalonieri S, Bertalot G, et. al.
EMBO reports
2016 vol: 17 (7) pp: 1061-80

Coronin 1C-free primary mouse fibroblasts exhibit robust rearrangements in the orientation of actin filaments, microtubules and intermediate filaments
Behrens J, Solga R, Ziemann A, Rastetter R, Berwanger C, et. al.
European Journal of Cell Biology
2016

Driven Translocation of Polynucleotides Through an Aerolysin Nanopore
Cao C, Yu J, Wang Y, Ying Y, Long Y, et. al.
Analytical Chemistry
2016 vol: 88 (10) pp: 5046-5049

Aurora A drives early signalling and vesicle dynamics during T-cell activation
Blas-Rus N, Bustos-Morán E, Pérez de Castro I, de Cárcer G, Borroto A, et. al.
Nature Communications
2016 vol: 7 pp: 11389

Morphotype-specific effector functions of Cryptococcus neoformans PUM1
Kaur J, Panepinto J
Scientific reports
2016 vol: 6 pp: 23638

In Vitro Investigation of Influences of Chitosan Nanoparticles on Fluorescein Permeation into Alveolar Macrophages
Chachuli S, Nawaz A, Shah K, Naharudin I, Wong T, et. al.
Pharmaceutical Research
2016 vol: 33 (6) pp: 1497-1508

HDAC6 regulates the dynamics of lytic granules in cytotoxic T lymphocytes
Núñez-Andrade N, Iborra S, Trullo A, Moreno-Gonzalo O, Calvo E, et. al.
Journal of cell science
2016 vol: 129 (7) pp: 1305-11

Disease causing mutations in inverted formin 2 regulate its binding to G-actin, F-actin capping protein (CapZ α-1) and profilin 2
Rollason R, Wherlock M, Heath J, Heesom K, Saleem M, et. al.
Bioscience reports
2016 vol: 36 (1) pp: e00302

Nuclear trapping through inhibition of exosomal export by indomethacin increases cytostatic efficacy of doxorubicin and pixantrone
Koch, Raphael;  Aung, Thiha;  Vogel, Daniel;  Chapuy, Bjoern;  Wenzel, Dirk et al. (2015)
Clin. Cancer Res. p. 1078-0432.CCR-15-0577-

Insulin Regulates the Activity of the High-Affinity Choline Transporter CHT
Fishwick, Katherine J.;  Rylett, R. Jane (2015)
PLOS ONE vol. 10 (7) p. e0132934

Regions outside of conserved PxxPxR motifs drive the high affinity interaction of GRB2 with SH3 domain ligands
Bartelt, Rebekah R.;  Light, Jonathan;  Vacaflores, Aldo;  Butcher, Alayna;  Pandian, Madhana et al. (2015)
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research vol. 1853 (10) p. 2560-2569

A STIM2 splice variant negatively regulates store-operated calcium entry
Miederer, Anna-Maria;  Alansary, Dalia;  Schwär, Gertrud;  Lee, Po-Hsien;  Jung, Martin et al. (2015)
Nature Communications vol. 6

Connection between the conformation and emission properties of poly[2-methoxy-5-(2′-ethyl-hexyloxy)-1,4-phenylene vinylene] single molecules during thermal annealing
Ou, Jiemei;  Yang, Yuzhao;  Lin, Wensheng;  Yuan, Zhongke;  Gan, Lin et al. (2015)
Applied Physics Letters    vol. 106 (12) p. 123304

Facilitation of Orai3 targeting and store-operated function by Orai1
Alansary, Dalia;  Bogeski, Ivan;  Niemeyer, Barbara A. (2015)
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research vol. 1853 (7) p. 1541-1550

GRB2 Nucleates T Cell Receptor-Mediated LAT Clusters That Control PLC-γ1 Activation and Cytokine Production
Bilal M Houtman J (2015)
Frontiers in Immunology vol: 6

Myosin VI deafness mutation prevents the initiation of processive runs on actin
Pylypenko O Song L Shima A Yang Z Houdusse A Sweeney H (2015)
Proceedings of the National Academy of Sciences vol: 112 (11) pp: E1201-E1209

PDK1 regulates focal adhesion disassembly by modulating endocytosis of αvβ3 integrin
di Blasio, Laura;  Gagliardi, Paolo Armando;  Puliafito, Alberto;  Sessa, Roberto;  Seano, Giorgio et al. (2015)
J. Cell Sci. vol. 128 (5) p. 863-877

Site specific phosphorylation of kindlin-3 regulates its capacity to control cellular responses mediated by integrin αIIbβ3
Bialkowska, Katarzyna;  Byzova, Tatiana V.;  Plow, Edward F. (2015)
J. Biol. Chem. p. M114.634436-

Multiplex single-molecule interaction profiling of DNA-barcoded proteins
Gu, Liangcai;  Li, Chao;  Aach, John;  Hill, David E;  Vidal, Marc et al. (2014)
Nature vol. 515 (7528)

PDZK1/NHERF3 Differentially Regulates Corticotropin-releasing Factor Receptor 1 and Serotonin 2A Receptor Signaling and Endocytosis
Walther, Cornelia;  Caetano, Fabiana A.;  Dunn, Henry A.;  Ferguson, Stephen S.G. (2015)
Cellular Signalling vol. 27 (3) p. 519-531

Membrane-cytoskeleton dynamics in the course of mast cell activation
Pavel Dráber, Petr Dráber (2015)
Methods in molecular biology 1220 p. 219-37

Multiplex single-molecule interaction profiling of DNA-barcoded proteins
Liangcai Gu, Chao Li, John Aach, David E. Hill, Marc Vidal, George M. Church (2014)
Nature 515 (7528) p. 554-557

Exosome poly-ubiquitin inhibits platelet activation, downregulates CD36 and inhibits pro-atherothombotic cellular functions
S Srikanthan, W Li, R L Silverstein, T M McIntyre (2014)
Journal of thrombosis and haemostasis : JTH 12 (11) p. 1906-17

PDK1-mediated activation of MRCKα regulates directional cell migration and lamellipodia retraction
Paolo Armando Gagliardi, Laura di Blasio, Alberto Puliafito, Giorgio Seano, Roberto Sessa, Federica Chianale, Thomas Leung, Federico Bussolino, Luca Primo (2014)
The Journal of cell biology 206 (3) p. 415-34

The large GTPase Mx1 is involved in apical transport of MDCK cells
Florian Hoff, Christoph Greb, Christina Hollmann, Ellena Hönig, Ralf Jacob (2014)
Traffic

Enhanced single molecule fluorescence of conjugated polymer poly(3-hexylthiophene) on silver-nanocubes
Jiemei Ou, Yuzhao Yang, Zhongke Yuan, Yu Zhang, Lin Gan, Xudong Chen (2014)
Synthetic Metals 195 p. 9-15

CLN3 Deficient Cells Display Defects in the ARF1-Cdc42 Pathway and Actin-Dependent Events
Mark L Schultz, Luis Tecedor, Colleen S Stein, Mark A Stamnes, Beverly L Davidson (2014)
PloS one 9 (5) p. e96647

Interactions of the protein-tyrosine phosphatase-α with the FAT domain of focal adhesion kinase are involved in IL-1 signaling in fibroblasts
Qin Wang, Yongqiang Wang, Dominik Fritz, Dhaarmini Rajshankar, Gregory P Downey, Christopher A McCulloch (2014)
The Journal of biological chemistry p. M113.540294-

Miro-1 links mitochondria and microtubule dynein motors to control lymphocyte migration and polarity
Giulia Morlino, Olga Barreiro, Francesc Baixauli, Javier Robles-Valero, José M González-Granado, Ricardo Villa-Bellosta, Jesús Cuenca, Carlos O Sánchez-Sorzano, Esteban Veiga, Noa B Martín-Cófreces, Francisco Sánchez-Madrid (2014)
Molecular and cellular biology p. MCB.01177-13-

Activated PLC-γ1 is catalytically induced at LAT but activated PLC-γ1 is localized at both LAT- and TCR-containing complexes
Noemi Cruz-Orcutt, Aldo Vacaflores, Sean F. Connolly, Stephen C. Bunnell, Jon C.D. Houtman (2014)
Cellular Signalling

Functional Cooperation of Metabotropic Adenosine and Glutamate Receptors Regulates Postsynaptic Plasticity in the Cerebellum
Y. Kamikubo, T. Shimomura, Y. Fujita, T. Tabata, T. Kashiyama, T. Sakurai, K. Fukurotani, M. Kano (2013)
Journal of Neuroscience 33 (47) p. 18661-18671

CLN3 Loss Disturbs Membrane Microdomain Properties and Protein Transport in Brain Endothelial Cells
Luis Tecedor, Colleen S Stein, Mark L Schultz, Hany Farwanah, Konrad Sandhoff, Beverly L Davidson (2013)
The Journal of neuroscience 33 (46) p. 18065-79

Focal Adhesion Kinase Negatively Regulates Lck Function Downstream of the T Cell Antigen Receptor
N. M. Chapman, S. F. Connolly, E. L. Reinl, J. C. D. Houtman (2013)
The Journal of Immunology p. 1301587

The GTPase-Activating Protein RN-tre Controls Focal Adhesion Turnover and Cell Migration
Andrea Palamidessi, Emanuela Frittoli, Nadia Ducano, Nina Offenhauser, Sara Sigismund, Hiroaki Kajiho, Dario Parazzoli, Amanda Oldani, Marco Gobbi, Guido Serini, Pier Paolo Di Fiore, Giorgio Scita, Letizia Lanzetti (2013)
Current Biology

TRPM7 triggers Ca2+ sparks and invadosome formation in neuroblastoma cells
Daan Visser, Michiel Langeslag, Katarzyna M. Kedziora, Jeffrey Klarenbeek, Alwin Kamermans, F. David Horgen, Andrea Fleig, Frank N. van Leeuwen, Kees Jalink (2013)
Cell Calcium

Seeded fibrillation as molecular basis of the species barrier in human prion diseases
Lars Luers, Oliver Bannach, Jan Stöhr, Michael Marius Wördehoff, Martin Wolff, Luitgard Nagel-Steger, Detlev Riesner, Dieter Willbold, Eva Birkmann (2013)
PloS one 8 (8) p. e72623

Dependence of Immunoglobulin Class Switch Recombination in B Cells on Vesicular Release of ATP and CD73 Ectonucleotidase Activity
Francesca Schena, Stefano Volpi, Caterina Elisa Faliti, Federica Penco, Spartaco Santi, Michele Proietti, Ursula Schenk, Gianluca Damonte, Annalisa Salis, Marta Bellotti, Franco Fais, Claudya Tenca, Marco Gattorno, Hermann Eibel, Marta Rizzi, Klaus Warnatz, Marco Idzko, Cemil Korcan Ayata, Mirzokhid Rakhmanov, Thierry Galli, Alberto Martini, Marco Canossa, Fabio Grassi, Elisabetta Traggiai (2013)
Cell Reports

Kindlin-1 Regulates Integrin Dynamics and Adhesion Turnover
Coert Margadant, Maaike Kreft, Giovanna Zambruno, Arnoud Sonnenberg (2013)
PLoS ONE 8 (6) p. e65341

PH-dependent recycling of galectin-3 at the apical membrane of epithelial cells
Tamara Straube, Tobias von Mach, Ellena Hönig, Christoph Greb, Dominik Schneider, Ralf Jacob (2013)
Traffic (Copenhagen, Denmark)

Uncoupling of mRNA synthesis and degradation impairs adaptation to host-temperature in Cryptococcus neoformans
Amanda L M Bloom, J T Graham Solomons, Virginia E Havel, John C Panepinto (2013)
Molecular microbiology

Barcoding cells using cell-surface programmable DNA-binding domains
Prashant Mali, John Aach, Je-Hyuk Lee, Daniel Levner, Lisa Nip, George M Church (2013)
Nature Methods

Spatial Coupling of JNK Activation to the B Cell Antigen Receptor by Tyrosine-Phosphorylated Ezrin
Neetha Parameswaran, Gospel Enyindah-Asonye, Nayer Bagheri, Neilay B Shah, Neetu Gupta (2013)
Journal of immunology p. jimmunol.1201292-

CD81 regulates cell migration through its association with Rac GTPase
Emilio Tejera, Vera Rocha-Perugini, Soraya López-Martín, Daniel Pérez-Hernández, Alexia I Bachir, Alan Rick Horwitz, Jesús Vázquez, Francisco Sánchez-Madrid, María Yáñez-Mo (2012)
Molecular biology of the cell p. mbc.E12-09-0642-

Mutations of the Ca2+-sensing Stromal Interaction Molecule STIM1 regulate Ca2+ influx by altered oligomerization of STIM1 and by destabilization of the Ca2+ channel Orai1
Tatiana Kilch, Dalia Alansary, Martin Peglow, Kathrin Doerr, Grigori Rychkov, Heiko Rieger, Christine Peinelt, Barbara A Niemeyer (2012)
The Journal of biological chemistry p. M112.417246-

End-binding protein 1 controls signal propagation from the T cell receptor
Noa B Martín-Cófreces, Francesc Baixauli, María J López, Diana Gil, Alicia Monjas, Balbino Alarcón, Francisco Sánchez-Madrid (2012)
The EMBO Journal

Total Internal Reflection Fluorescence (TIRF) Microscopy for Real-Time Imaging of Nanoparticle-Cell Plasma Membrane Interaction
Ladan Parhamifar, S Moein Moghimi (2012)
Methods in molecular biology (Clifton, N.J.) 906 p. 473-82

TβRIII/β-arrestin2 regulates integrin α5β1 trafficking, function, and localization in epithelial cells
K Mythreye, E H Knelson, C E Gatza, M L Gatza, G C Blobe (2012)
Oncogene

STIM1 SAM domain mutants regulate CRAC by altering oligomerization and by destabilizing Orai1
T. Kilch, D. Al-Ansary, M. Peglow, G. Rychkov, H. Rieger, C. Peinelt and B. A. Niemeyer (2012)

Oligomers of the ATPase EHD2 confine caveolae to the plasma membrane through association with actin
Miriam Stoeber, Ina Karen Stoeck, Christine Hänni, Christopher Karl Ernst Bleck, Giuseppe Balistreri, Ari Helenius (2012)
The EMBO journal

The novel formin FMNL3 is a cytoskeletal regulator of angiogenesis.
Clare Hetheridge, Alice N Scott, Rajeeb K Swain, John W Copeland, Henry N Higgs, Roy Bicknell, Harry Mellor (2012)
Journal of cell science p. jcs.091066-

TI-VAMP/VAMP7 is the snare of secretory lysosomes contributing to ATP secretion from astrocytes
Claudia Verderio, Cinzia Cagnoli, Matteo Bergami, Maura Francolini, Ursula Schenk, Alessio Colombo, Loredana Riganti, Carolina Frassoni, Emanuela Zuccaro, Lydia Danglot, Claire Wilhelm, Thierry Galli, Marco Canossa, Michela Matteoli (2011) oli
Biology of the Cell

Recruitment of vimentin to the cell surface by β3 integrin and plectin mediates adhesion strength.
Ramona Bhattacharya, Annette M Gonzalez, Phillip J Debiase, Humberto E Trejo, Robert D Goldman, Frederick W Flitney, Jonathan C R Jones (2009)
Journal of cell science 122 (Pt 9) p. 1390-400

Localization imaging using blinking quantum dots.
Fan-Ching Chien, Chiung Wen Kuo, Peilin Chen (2011)
The Analyst 136 (8) p. 1608-13

Ascorbate and endocytosed Motexafin gadolinium induce lysosomal rupture
Carsten Berndt, Tino Kurz, Sarah Bannenberg, Ralf Jacob, Arne Holmgren and Ulf T. Brunk
Cancer Letters, Volume 307, Issue 2, 28 August 2011, Pages 119-123, doi:10.1016/j.canlet.2011.03.023

Synthetic Protein Targeting by the Intrinsic Biorecognition Functionality of Poly(ethylene glycol) Using PEG Antibodies as Biohybrid Molecular Adaptors.
Janne T Hyotyla, Jie Deng, Roderick Y H Lim (2011)
ACS nano 5 (6) p. 5180-7

Exploring the Formation of Focal Adhesions on Patterned Surfaces Using Super-Resolution Imaging.
Fan-Ching Chien, Chiung Wen Kuo, Zong-Han Yang, Di-Yen Chueh, Peilin Chen (2011)
Small (Weinheim an der Bergstrasse, Germany) 7 (20) p. 2906-13

Trafficking of galectin-3 through endosomal organelles of polarized and non-polarized cells
Dominik Schneider, Christoph Greb, Annett Koch, Tamara Straube, Alexandra Ellia, Delphine Delacour and Ralf Jacob
European Journal of Cell Biology, Volume 89, Issue 11, November 2010, Pages 788-798, doi:10.1016/j.ejcb.2010.07.001

Photoswitching of E222Q GFP mutants: "concerted" mechanism of chromophore isomerization and protonation.
Stefania Abbruzzetti, Ranieri Bizzarri, Stefano Luin, Riccardo Nifosì, Barbara Storti, Cristiano Viappiani, Fabio Beltram (2010)
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology 9 (10) p. 1307-19

Autoimmune kidney disease and impaired engulfment of apoptotic cells in mice with macrophage peroxisome proliferator-activated receptor gamma or retinoid X receptor alpha deficiency.
Tamás Roszer, María P Menéndez-Gutiérrez, Martina I Lefterova, Daniel Alameda, Vanessa Núñez, Mitchell A Lazar, Thierry Fischer, Mercedes Ricote (2011)
Journal of immunology (Baltimore, Md. : 1950) 186 (1) p. 621-31

A microfluidic imaging chamber for the direct observation of chemotactic transmigration.
Mark T Breckenridge, Thomas T Egelhoff, Harihara Baskaran (2010)
Biomedical microdevices 12 (3) p. 543-53

Vinculin nucleates actin polymerization and modifies actin filament structure.
Kuo-Kuang Wen, Peter A Rubenstein, Kris A DeMali (2009)
The Journal of biological chemistry 284 (44) p. 30463-73

The mitochondrial fission factor dynamin-related protein 1 modulates T-cell receptor signalling at the immune synapse.
Francesc Baixauli, Noa B Martín-Cófreces, Giulia Morlino, Yolanda R Carrasco, Carmen Calabia-Linares, Esteban Veiga, Juan M Serrador, Francisco Sánchez-Madrid (2011)
The EMBO journal 30 (7) p. 1238-50

Bi-modal regulation of a formin by srGAP2.
Frank M Mason, Ernest G Heimsath, Henry N Higgs, Scott H Soderling (2011)
The Journal of biological chemistry 286 (8) p. 6577-86

Targeted nuclear delivery using peptide-coated quantum dots.
Chiung-Wen Kuo, Di-Yen Chueh, Narendra Singh, Fan-Ching Chien, Peilin Chen (2011)
Bioconjugate chemistry 22 (6) p. 1073-80

Cargo binding induces dimerization of myosin VI.
Denis Phichith, Mirko Travaglia, Zhaohui Yang, Xiaoyan Liu, Alan B Zong, Daniel Safer, H Lee Sweeney (2009)
Proceedings of the National Academy of Sciences of the United States of America 106 (41) p. 17320-4

Ccr4 promotes resolution of the endoplasmic reticulum stress response during host temperature adaptation in Cryptococcus neoformans.
Virginia E Havel, Nathan K Wool, David Ayad, Kurtis M Downey, Christabel F Wilson, Peter Larsen, Julianne T Djordjevic, John C Panepinto (2011)
Eukaryotic cell 10 (7) p. 895-901

Phosphorylated filamin A regulates actin-linked caveolae dynamics.
Olivia Muriel, Asier Echarri, Christian Hellriegel, Dácil M Pavón, Leonardo Beccari, Miguel A Del Pozo (2011)
Journal of cell science 124 (Pt 16) p. 2763-76

Conformational switching in ezrin regulates morphological and cytoskeletal changes required for B cell chemotaxis.
Neetha Parameswaran, Ken Matsui, Neetu Gupta (2011)
Journal of immunology (Baltimore, Md. : 1950) 186 (7) p. 4088-97

Proteomics analysis of the ezrin interactome in B cells reveals a novel association with Myo18aa.
Ken Matsui, Neetha Parameswaran, Nayer Bagheri, Belinda Willard, Neetu Gupta (2011)
Journal of proteome research 10 (9) p. 3983-92

Persistent cAMP-signals triggered by internalized G-protein-coupled receptors.
Davide Calebiro, Viacheslav O Nikolaev, Maria Cristina Gagliani, Tiziana de Filippis, Christian Dees, Carlo Tacchetti, Luca Persani, Martin J Lohse (2009)
PLoS biology 7 (8) p. e1000172

Biogenesis of caveolae: stepwise assembly of large caveolin and cavin complexes.
Arnold Hayer, Miriam Stoeber, Christin Bissig, Ari Helenius (2010)
Traffic (Copenhagen, Denmark) 11 (3) p. 361-82

RON (MST1R) is a novel prognostic marker and therapeutic target for gastroesophageal adenocarcinoma.
Daniel V T Catenacci, Gustavo Cervantes, Soheil Yala, Erik A Nelson, Essam El-Hashani, Rajani Kanteti, Mohamed El Dinali, Rifat Hasina, Johannes Brägelmann, Tanguy Seiwert, Michele Sanicola, Les Henderson, Tatyana A Grushko, Olufunmilayo Olopade, Theodore Karrison, Yung-Jue Bang, Woo Ho Kim, Maria Tretiakova, Everett Vokes, David A Frank, Hedy L Kindler, Heather Huet, Ravi Salgia (2011)
Cancer biology & therapy 12 (1) p. 9-46

Microtubule remodelling is required for the front-rear polarity switch during contact inhibition of locomotion.
Shereen Kadir, Jonathan W Astin, Lubna Tahtamouni, Paul Martin, Catherine D Nobes (2011)
Journal of cell science 124 (Pt 15) p. 2642-53

Interaction between CD36 and Oxidized LDL Modulates Macrophage Cytoskeletal Functions: A Mechanism of Macrophage Trapping
Young Mi Park (2010)

Covalent quantum dot receptor linkage via the acyl carrier protein for single-molecule tracking, internalization, and trafficking studies
Monika Zelman-Femiak, Kun Wang, Kira V. Gromova, Petra Knaus, Gregory S. Harms (2010)
BioTechniques 49 (2) p. 574–579

In situ assembly of macromolecular complexes triggered by light.
Christian Grunwald, Katrin Schulze, Annett Reichel, Victor U Weiss, Dieter Blaas, Jacob Piehler, Karl-Heinz Wiesmüller, Robert Tampé (2010)
Proceedings of the National Academy of Sciences of the United States of America 107 (14) p. 6146-51

STIM1-directed reorganization of microtubules in activated mast cells.
Zuzana Hájková, Viktor Bugajev, Eduarda Dráberová, Stanislav Vinopal, Lubica Dráberová, Jirí Janácek, Petr Dráber, Pavel Dráber (2011)
Journal of immunology (Baltimore, Md. : 1950) 186 (2) p. 913-23