恰如中文意涵的 THUNDER - 閃雷, 晴天霹靂的聲勢, 在影像技術掀起數位澄清化的雷擊 !
發布日期：2020/12/30
THUNDER 就是寬場 (wide-field) 3D 螢光影像的即時澄清化技術結合超解析的先進影像系統. 超高解析的 3D 澄清螢光影像以即時的速率呈現給用戶, 是 THUNDER 閃雷的一擊效應 !!!
因為 THUNDER 影像澄清系統的推出, 其背後的智慧型澄清化運算技術, 可對厚的樣本, 如模式動物或生物器官, 生物組織, 到細胞內分子等級的顯微生物樣本, 如蛋白質,病毒. 以超高速的即時速率, 將澄清後的高清晰 3D 螢光影像, 即時呈現在使用者面前, 所有 3D 螢光成像, 呈現出原始樣本的清晰細節, 不會產生任何的失焦模糊影像. 所展現出來的3D螢光影像, 令人震驚，是如此的豔麗, 清晰, 超高景深的高對比螢光影像, 所取得解析, 逼近超解析的尺度, 其震撼的影像效果, 宛如天空中劃過一道閃雷, 晴天霹靂 ! THUNDER 從此重新定義了樣本的高速高品質 3D 螢光影像澄清處理系統 !!!
依照樣本型態, THUNDER 為了最佳的工作效率, 將顯微鏡系統做最佳化的規劃, 提出三個基本系統, 供用戶參考選用 :
THUNDER Imager Model Organism
全自動實體澄清影像系統
可在發育或分子生物學研究中對整個生物機體進行 3D 探索。
系統架構在 LEICA 實體螢光顯微鏡.
疊加圖像：封固在凹玻片上直徑約 150 µm 的類器官。分別通過使用高解析度物鏡 (FluoCombi III) 的體視顯微鏡 [左] 和 THUNDER Imager Model Organism (右) 成像。THUNDER Imager system 可呈現解析度更高、更銳利的圖像。THUNDER Imager Tissue
全自動組織澄清影像系統
通常用於腦科學, 神經系統科學和組織學研究中的 3D 組織切片進行即時螢光3D成像。
架構在 LEICA 正立螢光顯微鏡
劍水蚤屬細胞核 (綠色)，乙醯化微管蛋白 (紅色)，血清素 (青綠色)。總體積為 332 x 332 x 84 µm³ 的三色圖像堆疊，每種顏色包括 305 個 z 平面。使用的物鏡為 HC PL FLUOTAR 40x/1.30 OIL。
THUNDER Imager Live Cell & 3D Cell Culture & 3D Assay
全自動活細胞澄清影像系統
可為您提供適用于先進 3D 細胞培養試驗的解決方案，無論您想要研究的是幹細胞、球狀細胞團或是類器官。
架構在 LEICA DMi8 倒立螢光顯微鏡
對照組瑞士成年小鼠全組織視網膜，顯示 Iba1 + 小膠質細胞（Alexa Fluor® 488 綠色螢光染色）和 Brn3a + 視網膜神經節細胞（Alexa Fluor® 594 紅色螢光染色）。 圖片由西班牙 Murcia 大學的實驗眼科學小組提供。
THUNDER Imager 的核心技術 :
採用 LEICA 創新智慧型 g螢光影像澄清化運算技術 (Computational Clearing)， 能夠即時 (real time ) 有效去除非焦平面的模糊信息, 宛如共軛焦成像, 都取得同一焦平面的影像信息, THUNDER 以全面優化澄清化螢光3D影像, 系統的高度靈敏度可確保低光毒性和低螢光淬滅，得以實現超高解析的 3D 澄清化螢光影像.
THUNDER Imager Live Cell 和 THUNDER Imager 3D Cell Culture 能夠在長時間實驗條件下, 因為有主動式自動對焦組成, 始終將焦點保持在活細胞的焦平面上，實現可靠的圖像資料攝取。
由於漂移、形態變化或生長，活細胞成像通常十分棘手。漂移是由於振動、機械蠕變或溫度波動導致的。漂移和細胞變化都會降低所攝取圖像資料的可靠性，因為對焦是一個問題。但得益于自我調整調焦控制 (AFC)、閉環對焦和軟體自動對焦功能，THUNDER Imager Live Cell 和 THUNDER Imager 3D Cell Culture 能夠為您的多孔實驗可靠地保持聚焦。
將 THUNDER 的離焦模糊去除功能與 TIRF 的優勢相結合。 對於細胞表面的動力學過程，全內反射螢光顯微鏡可提供出色的信號背景分離。
此處兩個視頻顯示用 GFP-GRINCH 表達人胰島素原的 ins-1 細胞。 在細胞培養中加入 KCL 後，產生胰島素的細胞去極化，可以觀察到殘留的胰島素與細胞質膜融合。THUNDER Imager Live Cell 與 TIRF 相結合，能夠以出色的清晰度、速度和成像參數控制對敏感的樣本成像。
THUNDER 可提供的優勢 :
1). 所獲得的 3D 影像, 是通過澄清化, 呈現高解析的有意義結構, 幫助科研取得好成果.
2). 高通量，可實現更好的統計和工作流程效率
3). 從你的顯微鏡為基礎, 架構唯一超解析的 3D 螢光澄清化影像系統, 效益與效率是值得投資的.
4). 從優化影像系統的處理條件, 可以更優化樣本的生理條件，獲取有意義的結果
LEICA 為你解說 THUNDER 的重要性
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Biotechnology/Bioengineering
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Biochemistry
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Pharmacology
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