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STELLARIS 8 STED

LEICA 與 2014 年諾貝爾獎得主 Stefan Hell合作開發的 STED 超解析奈米螢光影像系統, 在共軛焦的優越性能上, 再把光學解析直接推到 3D超解析, 可達到數十奈米, 突破百年來的 ABBE 光學繞射極限. 此 STED直接光學的技術, 是超解析技術中最具實用.
 
STELLARIS 8 STED 在進一步在共軛焦掃描的 STED 系統中導入時脈頻譜技術, 延伸使用了高速分析螢光生命週期, 效益上可以降低光劑量, 減低光毒害造成的活細胞傷害, 同時把純光學解析推進到優於30 nm (lateral), 100 nm ( axial ).



 



STED 技術, 可以改善螢光光點的點擴散函數 ( PSF ), 使得光學解析得以提高, 達到奈米解析度.
 
STED 技術, 在共軛焦掃描的機制上, 輔以高功率的壓制雷射光, 讓螢光釋放區域縮小, 等同解析提高. 是一次性的共軛焦掃描, 即可同時得到超解析影像, 不用耗時耗力的長時間做影像後製運算 (如STORM, PALM 等技術)．
 
通過脈衝光源, 時間閘門控制技術, 螢光生命週期分析技術, STED 技術可以避免螢光漂白與細胞傷害, 直接運用到活細胞的長時間影像擷取. 同時取的純光學解析達30 nm, 可運用到多色螢光影像, 包括遠紅外光染劑的使用 ( 如SiR, SPY )
 










STED 3D 光路圖示


LEICA STELLARIS 8 STED 基本配置脈衝壓制雷射光源 ( 775 nm ), 必須搭配脈衝白光雷射與 AOBS 分光系統.
選配 592 nm CW 與 660 nm nm CW 雷射光源.
( 詳細請洽本公司專員 )

STED for Immunology: Living T cell in suspension. 3D reconstruction of confocal and STED stacks. Image courtesy: Marco Fritsche, Mathias Clausen and Christian Eggeling, MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, UK.​



STED for kidney disease research: 3D STED 775 deep nanoscopy of glomerulus in cleared kidney tissue immunostained for nephrin. Scale bar: 10 μm, z-depth color coded. Sample courtesy: David Unnersjö Jess, KTH, Stockholm, Sweden.







τ-STED 660 on COS7 cells immuno-stained for nuclear pores (NUPs). The primary antibody recognizes several proteins from the nuclear pore basket, thus the point-like structures observed. Only 2% of STED light reveals a great amount of additional detail.





STED for Developmental Biology: Single molecule in-situ hybridization (smFISH) of RNA in Drosophila embryo whole-mount preparation. Probes are directly labeled (no signal amplification). Top: Three color t-STED 775 captures the signal from hb CalFluor 610 (cyan), gt Quasar 670 (green), eve Quasar 705 (magenta). Bottom: confocal overview of the Drosophila embryo preparation. Sample courtesy: Tom Pettini, University of Manchester, UK. 





Pushing STED beyond its Limits with Lifetime
 
TauSTED combines the optical signals from STED and the physical information from fluorescence lifetime at confocal speeds. Outstanding STED resolution and image quality is achieved due to background removal, at gentle imaging conditions for extended STED acquisitions in time and space.
 
Benefits
Outstanding image quality: Selecting and removing the lifetime fingerprint of photons from background noise translates into images with excellent signal to noise.
Gentle live-cell imaging: The access to lifetime information at confocal speed protects specimens during long-time STED experiments at lower excitation and STED light doses.

STED for cell biology, multi-color live cell TauSTED 775 resolves the cytoskeleton network labeled with SiR-tubulin (Glow), and tracking vesicles labeled with CF594 (Cyan).




LEICA STED 專用高性能物鏡 (獨家設計)
 
 
STED WHITE 100x 油鏡
可提供最高的數值孔徑（NA 1.40）和 光學解析度，適合固定樣本的奈米尺度影像表像
 
STED WHITE 93x 甘油物鏡
適合深層STED奈米影像實驗，其具有電動校正環可快速準確地適應2D和3D的蓋玻片厚度，對樣本不均勻的補償, 溫度變化的補償.
 
STED WHITE 86x Water 水鏡,
適合用於活細胞與活體實驗。 motCORR技術使該鏡頭在活細胞和STED-FCS應用中成為強大的伴侶.






導航直觀的應用軟體, 提供自動化的簡易使用, 幾乎人人可以上手使用. 

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探索 STED Tau-Sense 超解析時脈頻譜技術 (影片)


從大師 Stefan Hell 的演講, 可以深入了解超解析的技術原理